BMRB Entry 15840

Name: Chemical Shift assignment of RhR2 from Rodobacter Sphaeroides, North east Structural Genomics Consortium Target SeR13
Published: 2008-07-24

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15840

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift assignment of RhR2 from Rodobacter Sphaeroides, North east Structural Genomics Consortium Target SeR13

Deposition date:

2008-06-28

Original release date:

2008-07-24

Authors:

Lee, Hsiau-Wei; Bansal, Sonal; Chen, Chen; Jiang, Mei; Maglaqui, Melissa; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Prestegard, James

Citation:

Citation: Lee, Hsiau-Wei; Bansal, Sonal; Montelione, Gaetano; Prestegard, James. "Solution Structure of RhR2" .

Assembly members:

Assembly members:
RhR2, polymer, 70 residues, 7148.213 Da.

Natural source:

Natural source: Common Name: Rhodobacter sphaeroides Taxonomy ID: 1063 Superkingdom: Bacteria Kingdom: not available Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RhR2: MRDMTEETRKDLPPEALRAL AEAEERRRRAKALDLPKEIG GRNGPEPVRFGDWEKKGIAI DFLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
RDCs
- RDC_list_1

Data type	Count
13C chemical shifts	215
15N chemical shifts	53
1H chemical shifts	308
residual dipolar couplings	19

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RhR2	1

Entities:

Entity 1, RhR2 70 residues - 7148.213 Da.

LEHHHHHH are at the C-terminal as part of His-Tag

1	MET	ARG	ASP	MET	THR	GLU	GLU	THR	ARG	LYS
2	ASP	LEU	PRO	PRO	GLU	ALA	LEU	ARG	ALA	LEU
3	ALA	GLU	ALA	GLU	GLU	ARG	ARG	ARG	ARG	ALA
4	LYS	ALA	LEU	ASP	LEU	PRO	LYS	GLU	ILE	GLY
5	GLY	ARG	ASN	GLY	PRO	GLU	PRO	VAL	ARG	PHE
6	GLY	ASP	TRP	GLU	LYS	LYS	GLY	ILE	ALA	ILE
7	ASP	PHE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQCTROSY	sample_2	anisotropic	sample_conditions_1

PDB	2K5K
GB	ABA80289 ABN77868 EGJ22606 EKX58174
REF	WP_009563153 WP_011338719 WP_043764254 YP_354190

BMRB Entry 15840

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	ARG	ASP	MET	THR	GLU	GLU	THR	ARG	LYS
2	ASP	LEU	PRO	PRO	GLU	ALA	LEU	ARG	ALA	LEU
3	ALA	GLU	ALA	GLU	GLU	ARG	ARG	ARG	ARG	ALA
4	LYS	ALA	LEU	ASP	LEU	PRO	LYS	GLU	ILE	GLY
5	GLY	ARG	ASN	GLY	PRO	GLU	PRO	VAL	ARG	PHE
6	GLY	ASP	TRP	GLU	LYS	LYS	GLY	ILE	ALA	ILE
7	ASP	PHE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	ARG	ASP	MET	THR	GLU	GLU	THR	ARG	LYS
2	ASP	LEU	PRO	PRO	GLU	ALA	LEU	ARG	ALA	LEU
3	ALA	GLU	ALA	GLU	GLU	ARG	ARG	ARG	ARG	ALA
4	LYS	ALA	LEU	ASP	LEU	PRO	LYS	GLU	ILE	GLY
5	GLY	ARG	ASN	GLY	PRO	GLU	PRO	VAL	ARG	PHE
6	GLY	ASP	TRP	GLU	LYS	LYS	GLY	ILE	ALA	ILE
7	ASP	PHE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	ARG	ASP	MET	THR	GLU	GLU	THR	ARG	LYS
2	ASP	LEU	PRO	PRO	GLU	ALA	LEU	ARG	ALA	LEU
3	ALA	GLU	ALA	GLU	GLU	ARG	ARG	ARG	ARG	ALA
4	LYS	ALA	LEU	ASP	LEU	PRO	LYS	GLU	ILE	GLY
5	GLY	ARG	ASN	GLY	PRO	GLU	PRO	VAL	ARG	PHE
6	GLY	ASP	TRP	GLU	LYS	LYS	GLY	ILE	ALA	ILE
7	ASP	PHE	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS