BMRB Entry 15834

Name: Solution NMR structure of FeoA protein from Chlorobium tepidum. Northeast Structural Genomics Consortium target CtR121
Published: 2008-07-24

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PDB ID: 2k5f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15834
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of FeoA protein from Chlorobium tepidum. Northeast Structural Genomics Consortium target CtR121

Deposition date:

2008-06-27

Original release date:

2008-07-24

Authors:

Eletsky, Alexander; Sathyamoorthy, Bharathwaj; Mills, Jeffrey; Zeri, Ana; Zhao, Li; Hamilton, Keith; Foote, Erica; Xiao, Rong; Nair, Rajesh; Baran, Michael; Swapna, G.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas

Citation:

Citation: Eletsky, Alexander; Sathyamoorthy, Bharathwaj; Mills, Jeffrey; Zeri, Ana; Zhao, Li; Hamilton, Keith; Foote, Erica; Xiao, Rong; Nair, Rajesh; Baran, Michael; Swapna, G.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR structure of FeoA protein from Chlorobium tepidum. Northeast Structural Genomics Consortium target CtR121" Proteins: Struct. Funct. Genet. ., .-..

Assembly members:

Assembly members:
FeoA_protein, polymer, 105 residues, 11872.900 Da.

Natural source:

Natural source: Common Name: Chlorobium tepidum Taxonomy ID: 1097 Superkingdom: Bacteria Kingdom: not available Genus/species: Chlorobium tepidum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FeoA_protein: MKLSELKAGDRAEVTSVAAE PAVRRRLMDLGLVRGAKLKV LRFAPLGDPIEVNCNGMLLT MRRNEAEGITVHILAGDEGH PHGWPGFRRRHRFGKRALEH HHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
coupling_constants
- coupling_constant_list_1

Data type	Count
13C chemical shifts	416
15N chemical shifts	104
1H chemical shifts	682
coupling constants	68

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FeoA_protein	1

Entities:

Entity 1, FeoA_protein 105 residues - 11872.900 Da.

8-residue C-terminal tag LEHHHHHH

1

MET

LYS

LEU

SER

GLU

LEU

LYS

ALA

GLY

ASP

2

ARG

ALA

GLU

VAL

THR

SER

VAL

ALA

GLU

3

PRO

ALA

VAL

ARG

LEU

MET

ASP

LEU

4

GLY

LEU

VAL

ARG

GLY

ALA

LYS

LEU

LYS

VAL

5

LEU

ARG

PHE

ALA

PRO

LEU

GLY

ASP

PRO

ILE

6

GLU

VAL

ASN

CYS

ASN

GLY

MET

LEU

THR

7

MET

ARG

ASN

GLU

ALA

GLU

GLY

ILE

THR

8

VAL

HIS

ILE

LEU

ALA

GLY

ASP

GLU

GLY

HIS

9

PRO

HIS

GLY

TRP

PRO

GLY

PHE

ARG

10

HIS

ARG

PHE

GLY

LYS

ARG

ALA

LEU

GLU

HIS

11

HIS

Samples:

NC: FeoA protein, [U-100% 13C; U-100% 15N], 1.26 mM; sodium azide 0.02%; DTT 100 mM; calcium chloride 5 mM; sodium chloride 100 mM; MES 20 mM; DSS 50 uM; H2O 90%; D2O, [U-100% 2H], 10%

NC5: FeoA protein, [U-5% 13C; U-100% 15N], 1.26 mM; sodium azide 0.02%; DTT 100 mM; calcium chloride 5 mM; sodium chloride 100 mM; MES 20 mM; DSS 50 uM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 115 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NC	isotropic	sample_conditions_1
2D 1H-13C HSQC	NC	isotropic	sample_conditions_1
3D HNCO	NC	isotropic	sample_conditions_1
3D CBCA(CO)NH	NC	isotropic	sample_conditions_1
3D HNCACB	NC	isotropic	sample_conditions_1
3D HBHA(CO)NH	NC	isotropic	sample_conditions_1
3D HCCH-TOCSY	NC	isotropic	sample_conditions_1
(4,3)D GFT HCCH-COSY ali	NC	isotropic	sample_conditions_1
(4,3)D GFT L-HCCH-COSY aro	NC	isotropic	sample_conditions_1
3D HNHA	NC	isotropic	sample_conditions_1
3D HNCO (hbond)	NC	isotropic	sample_conditions_1
3D 1H-15N,13Cali,13Caro NOESY	NC	isotropic	sample_conditions_1
2D 1H-13C CT-HSQC 28ms	NC5	isotropic	sample_conditions_1

Software:

VNMRJ v2.1B, Varian - collection

PROSA v6.0.2, Guntert - processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

XEASY v1.3.13, Bartels et al. - data analysis

AutoAssign v1.15.1, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CSI v2.0, Wishat and Sykes - data analysis

TALOS v2003.027.13.05, Cornilescu, Delaglio and Bax - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

PSVS v1.3, Bhattacharya and Montelione - validation

NMR spectrometers:

Varian INOVA 750 MHz

PDB	2K5F
GB	AAM71305
REF	NP_660963 WP_010931751