BMRB Entry 15832

Name: chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y5-receptor
Published: 2009-02-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15832

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y5-receptor

Deposition date:

2008-06-27

Original release date:

2009-02-13

Authors:

Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini

Citation:

Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy." J. Pept. Sci. 15, 184-191 (2009).
PubMed: 19125388

Assembly members:

Assembly members:
N-Y5, polymer, 51 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pUBK19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-Y5: MSFYSKQDYNMDLELDEYYN KTLATENNTAATRNSDFPVW DDYKSSVDDLQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
heteronucl_NOEs
- heteronuclear_noe_list_1

Data type	Count
15N chemical shifts	48
1H chemical shifts	48
heteronuclear NOE values	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-Y5	1

Entities:

Entity 1, N-Y5 51 residues - Formula weight is not available

1

MET

SER

PHE

TYR

SER

LYS

GLN

ASP

TYR

ASN

2

MET

ASP

LEU

GLU

LEU

ASP

GLU

TYR

ASN

3

LYS

THR

LEU

ALA

THR

GLU

ASN

THR

ALA

4

ALA

THR

ARG

ASN

SER

ASP

PHE

PRO

VAL

TRP

5

ASP

TYR

LYS

SER

VAL

ASP

LEU

6

GLN

Samples:

sample_1: N-Y5, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: N-Y5, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 310 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N NOE	sample_2	isotropic	sample_conditions_2

Software:

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz

DBJ	BAG74150 BAK62588
GB	AAC50623 AAC50741 AAC51295 AAG40773 AAH42416
REF	NP_001028005 NP_001266807 NP_006165 XP_003899379 XP_004040628
SP	Q15761
AlphaFold	Q15761