BMRB Entry 15831

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15831

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Title:
chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y2-receptor
Deposition date:
2008-06-27
Original release date:
2009-02-13
Authors:
Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini
Citation:

Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy."  J. Pept. Sci. 15, 184-191 (2009).
PubMed: 19125388

Assembly members:

Assembly members:
N-Y2, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUBK19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-Y2: MGPIGAEADENQTVEEMKVE QYGPQTTPRGELVPDPEPEL IDSTKLIEVQ

Data sets:
Data typeCount
15N chemical shifts43
1H chemical shifts43
heteronuclear NOE values43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Y21

Entities:

Entity 1, N-Y2 50 residues - Formula weight is not available

1   METGLYPROILEGLYALAGLUALAASPGLU
2   ASNGLNTHRVALGLUGLUMETLYSVALGLU
3   GLNTYRGLYPROGLNTHRTHRPROARGGLY
4   GLULEUVALPROASPPROGLUPROGLULEU
5   ILEASPSERTHRLYSLEUILEGLUVALGLN

Samples:

sample_1: N-Y2, [U-15N], 0.2 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: N-Y2, [U-15N], 0.4 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N NOEsample_2isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

DBJ BAG74149
GB AAA93170 AAB04120 AAB07760 AAC50281 AAC51115
REF NP_000901 NP_001012655 NP_001028004 XP_002832879 XP_003257918
SP P49146 Q5IS62 Q9GK74
AlphaFold Q5IS62 Q9GK74 P49146

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks