BMRB Entry 15830

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15830

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Title:
chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y1-receptor
Deposition date:
2008-06-27
Original release date:
2009-02-13
Authors:
Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini
Citation:

Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy."  J. Pept. Sci. 15, 184-191 (2009).
PubMed: 19125388

Assembly members:

Assembly members:
N-Y1, polymer, 40 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUBK19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-Y1: MNSTLFSQVENHSVHSNFSE KNAQLLAFENDDCHLPLAMI

Data sets:
Data typeCount
15N chemical shifts36
1H chemical shifts249
heteronuclear NOE values35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Y11

Entities:

Entity 1, N-Y1 40 residues - Formula weight is not available

1   METASNSERTHRLEUPHESERGLNVALGLU
2   ASNHISSERVALHISSERASNPHESERGLU
3   LYSASNALAGLNLEULEUALAPHEGLUASN
4   ASPASPCYSHISLEUPROLEUALAMETILE

Samples:

sample_1: N-Y1, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: N-Y1, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 6.4; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N NOEsample_2isotropicsample_conditions_2
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAD92474 BAG35472
GB AAA59920 AAA59947 AAA73215 AAG40771 AAH36657
REF NP_000900 NP_001028038 XP_002815306 XP_003257965 XP_003822577
SP P25929
AlphaFold P25929

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