BMRB Entry 15820
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15820
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NMR-STAR v3 text file.
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Citation: Wu, Zuopeng; Jia, Xinying; de la Cruz, Laura; Su, Xun-Cheng; Marzolf, Bruz; Troisch, Pamela; Zak, Daniel; Hamilton, Adam; Whittle, Belinda; Yu, Di; Sheahan, Daniel; Bertram, Edward; Alderem, Alan; Otting, Gottfried; Goodnow, Christopher; Hoyne, Gerard. "Memory T cell RNA rearrangement programmed by heterogeneous nuclear ribonucleoprotein hnRNPLL" Immunity 29, 863-875 (2008).
PubMed: 19100700
Assembly members:
RRM1, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETMCSIII
Entity Sequences (FASTA):
RRM1: MHHHHHHMLSTEGGGSHHKV
SVSPVVHVRGLCESVVEADL
VEALEKFGTICYVMMMPFKR
QALVEFENIDSAKECVTFAA
DVPVYIAGQQAFFNYSTSKR
ITRPGNTDDPSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 292 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RRM1 of hnRNPLL | 1 |
Entities:
Entity 1, RRM1 of hnRNPLL 112 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | MET | LEU | SER | ||||
| 2 | THR | GLU | GLY | GLY | GLY | SER | HIS | HIS | LYS | VAL | ||||
| 3 | SER | VAL | SER | PRO | VAL | VAL | HIS | VAL | ARG | GLY | ||||
| 4 | LEU | CYS | GLU | SER | VAL | VAL | GLU | ALA | ASP | LEU | ||||
| 5 | VAL | GLU | ALA | LEU | GLU | LYS | PHE | GLY | THR | ILE | ||||
| 6 | CYS | TYR | VAL | MET | MET | MET | PRO | PHE | LYS | ARG | ||||
| 7 | GLN | ALA | LEU | VAL | GLU | PHE | GLU | ASN | ILE | ASP | ||||
| 8 | SER | ALA | LYS | GLU | CYS | VAL | THR | PHE | ALA | ALA | ||||
| 9 | ASP | VAL | PRO | VAL | TYR | ILE | ALA | GLY | GLN | GLN | ||||
| 10 | ALA | PHE | PHE | ASN | TYR | SER | THR | SER | LYS | ARG | ||||
| 11 | ILE | THR | ARG | PRO | GLY | ASN | THR | ASP | ASP | PRO | ||||
| 12 | SER | GLY |
Samples:
sample_1: RRM1, [U-100% 13C; U-100% 15N], 0.5-0.8 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_2: RRM1, [U-100% 15N], 0.5-0.8 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Related Database Links:
| DBJ | BAB28521 BAC28858 BAE02506 BAE27500 BAE42243 |
| EMBL | CAH56358 |
| GB | AAH08217 AAH12849 AAH17480 AAI23452 AAN76189 |
| REF | NP_001070368 NP_001136122 NP_001267087 NP_612403 NP_659051 |
| SP | Q8WVV9 Q921F4 |
| TPG | DAA24711 |
| AlphaFold | Q8WVV9 Q921F4 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks