BMRB Entry 15817
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15817
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Citation: Siemer, Ansgar; McDermott, Ann. "Solid-State NMR on a Type III Antifreeze Protein in the Presence of Ice" J. Am. Chem. Soc. 130, 17394-17399 (2008).
PubMed: 19053456
Assembly members:
type_III_AFP_isoform_HPLC-12, polymer, 74 residues, Formula weight is not available
Natural source: Common Name: ocean pout Taxonomy ID: 8199 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Macrozoarces americanus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-20b
Entity Sequences (FASTA):
type_III_AFP_isoform_HPLC-12: MNQASVVANQLIPINTALTL
VMMRSEVVTPVGIPAEDIPR
LVSMQVNRAVPLGTTLMPDM
VKGYAALEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 279 |
| 15N chemical shifts | 60 |
| 1H chemical shifts | 352 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | monomer | 1 |
Entities:
Entity 1, monomer 74 residues - Formula weight is not available
| 1 | MET | ASN | GLN | ALA | SER | VAL | VAL | ALA | ASN | GLN | ||||
| 2 | LEU | ILE | PRO | ILE | ASN | THR | ALA | LEU | THR | LEU | ||||
| 3 | VAL | MET | MET | ARG | SER | GLU | VAL | VAL | THR | PRO | ||||
| 4 | VAL | GLY | ILE | PRO | ALA | GLU | ASP | ILE | PRO | ARG | ||||
| 5 | LEU | VAL | SER | MET | GLN | VAL | ASN | ARG | ALA | VAL | ||||
| 6 | PRO | LEU | GLY | THR | THR | LEU | MET | PRO | ASP | MET | ||||
| 7 | VAL | LYS | GLY | TYR | ALA | ALA | LEU | GLU | HIS | HIS | ||||
| 8 | HIS | HIS | HIS | HIS |
Samples:
sample_1: H2O 90%; D2O 10%; sodium phosphate 10 mM; type III AFP isoform HPLC-12, [U-100% 13C; U-100% 15N], 1 mg
sample_conditions_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 297 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v1.3, Bruker Biospin - collection, processing
CARA v1.8.4, Keller and Wuthrich - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks