BMRB Entry 15811

Name: Solution structure of 30S ribosomal protein S27A from Thermoplasma acidophilum
Published: 2009-04-23

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PDB ID: 2k4x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15811
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of 30S ribosomal protein S27A from Thermoplasma acidophilum

Deposition date:

2008-06-20

Original release date:

2009-04-23

Authors:

Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Arrowsmith, Cheryl

Citation:

Citation: Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Semest, Anthony; Arrowsmith, Cheryl. "Solution structure of 30S ribosomal protein S27A from Thermoplasma acidophilum/Northeast Structural Genomics Consortium Target TaT88/Ontario Center for Structural Proteomics target ta1093" .

Assembly members:

Assembly members:
30S_ribosomal_protein_S27A, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Thermoplasma acidophila Taxonomy ID: 2303 Superkingdom: Archaea Kingdom: not available Genus/species: Thermoplasma acidophilum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
30S_ribosomal_protein_S27A: MQKRELYEIADGKLVRKHRF CPRCGPGVFLAEHADRYSCG RCGYTEFKKAKKSKS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	224
15N chemical shifts	51
1H chemical shifts	353

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	30S_ribosomal	1
2	ZINC ION	2

Entities:

Entity 1, 30S_ribosomal 55 residues - Formula weight is not available

1

MET

GLN

LYS

ARG

GLU

LEU

TYR

GLU

ILE

ALA

2

ASP

GLY

LYS

LEU

VAL

ARG

LYS

HIS

ARG

PHE

3

CYS

PRO

ARG

CYS

GLY

PRO

GLY

VAL

PHE

LEU

4

ALA

GLU

HIS

ALA

ASP

ARG

TYR

SER

CYS

GLY

5

ARG

CYS

GLY

TYR

THR

GLU

PHE

LYS

ALA

6

LYS

SER

LYS

SER

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_2: 30S ribosomal protein S27A, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; ZnSO4 10 uM; DTT, [U-100% 2H], 10 mM; sodium azide 0.01%; benzamidine 10 mM; D2O, [U-100% 2H], 100%

sample_1: 30S ribosomal protein S27A, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; ZnSO4 10 uM; DTT, [U-100% 2H], 10 mM; sodium azide 0.01%; benzamidine 10 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.95, Goddard - data analysis, peak picking

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 800 MHz

PDB	2K4X
EMBL	CAC12221
REF	WP_010901503
SP	Q9HJ78
AlphaFold	Q9HJ78