BMRB Entry 15800
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15800
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Nesmelova, Irina; Pang, Mabel; Baum, Linda; Mayo, Kevin. "1H, 13C, and 15N backbone and side-chain chemical shift assignments for the 29 kDa human galectin-1 protein dimer" Biomol. NMR Assignments 2, 203-205 (2008).
PubMed: 19636905
Assembly members:
galectin-1, polymer, 134 residues, Formula weight is not available
LAT, non-polymer, 342.296 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEMEX
Entity Sequences (FASTA):
galectin-1: ACGLVASNLNLKPGECLRVR
GEVAPDAKSFVLNLGKDSNN
LCLHFNPRFNAHGDANTIVC
NSKDGGAWGTEQREAVFPFQ
PGSVAEVCITFDQANLTVKL
PDGYEFKFPNRLNLEAINYM
AADGDFKIKCVAFD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 371 |
| 15N chemical shifts | 141 |
| 1H chemical shifts | 822 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit1 | 1 |
| 2 | subunit2 | 1 |
| 3 | ligand1 | 2 |
| 4 | ligand2 | 2 |
Entities:
Entity 1, subunit1 134 residues - Formula weight is not available
| 1 | ALA | CYS | GLY | LEU | VAL | ALA | SER | ASN | LEU | ASN | ||||
| 2 | LEU | LYS | PRO | GLY | GLU | CYS | LEU | ARG | VAL | ARG | ||||
| 3 | GLY | GLU | VAL | ALA | PRO | ASP | ALA | LYS | SER | PHE | ||||
| 4 | VAL | LEU | ASN | LEU | GLY | LYS | ASP | SER | ASN | ASN | ||||
| 5 | LEU | CYS | LEU | HIS | PHE | ASN | PRO | ARG | PHE | ASN | ||||
| 6 | ALA | HIS | GLY | ASP | ALA | ASN | THR | ILE | VAL | CYS | ||||
| 7 | ASN | SER | LYS | ASP | GLY | GLY | ALA | TRP | GLY | THR | ||||
| 8 | GLU | GLN | ARG | GLU | ALA | VAL | PHE | PRO | PHE | GLN | ||||
| 9 | PRO | GLY | SER | VAL | ALA | GLU | VAL | CYS | ILE | THR | ||||
| 10 | PHE | ASP | GLN | ALA | ASN | LEU | THR | VAL | LYS | LEU | ||||
| 11 | PRO | ASP | GLY | TYR | GLU | PHE | LYS | PHE | PRO | ASN | ||||
| 12 | ARG | LEU | ASN | LEU | GLU | ALA | ILE | ASN | TYR | MET | ||||
| 13 | ALA | ALA | ASP | GLY | ASP | PHE | LYS | ILE | LYS | CYS | ||||
| 14 | VAL | ALA | PHE | ASP |
Entity 2, ligand1 - C12 H22 O11 - 342.296 Da.
| 1 | LAT |
Samples:
sample_1: galectin-1, [U-100% 13C; U-100% 15N], 0.8-1 mM; lactose 2 mM; potassium phosphate buffer 20 mM; DTT 8 mM
sample_2: galectin-1, [U-100% 13C; U-100% 15N], 0.8-1 mM; lactose 2 mM; potassium phosphate buffer 20 mM; DTT 8 mM
sample_conditions_1: ionic strength: 20 mM; pH: 5.2; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 17184 |
| PDB | |
| DBJ | BAC77389 BAG35095 BAG72608 |
| EMBL | CAA32938 CAA33328 CAG30397 CAH92239 CAK54541 |
| GB | AAA36170 AAB00777 AAH01693 AAH20675 AAP35421 |
| PRF | 1409286A 1713410A |
| REF | NP_001126310 NP_001135409 NP_001162098 NP_002296 XP_001162104 |
| SP | P09382 Q5R7M1 |
| AlphaFold | P09382 Q5R7M1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks