BMRB Entry 15796

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15796
MolProbity Validation Chart

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Title:
E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity
Deposition date:
2008-06-06
Original release date:
2008-11-14
Authors:
Huang, Anding; De Jong, Rob; Hans, Wienk; Sebastiaan, Winkler; Marc, Timmers; Rolf, Boelens
Citation:

Citation: Huang, Anding; De Jong, Rob; Wienk, Hans; Winkler, Sebastiaan; Timmers, Marc; Boelens, Rolf. "E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity"  J. Mol. Biol. 385, 507-519 (2008).
PubMed: 18996392

Assembly members:

Assembly members:
E2-c-Cbl, polymer, 83 residues, 9387.704 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLICHISGST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E2-c-Cbl: GSLQDHIKVTQEQYELYCEM GSTFQLCKICAENDKDVKIE PCGHLMCTSCLTSWQESEGQ GCPFCRCEIKGTEPIVVDPF DPR

Data typeCount
13C chemical shifts403
15N chemical shifts93
1H chemical shifts590

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1E2-c-Cbl1
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, E2-c-Cbl 83 residues - 9387.704 Da.

N-terminal GSL is a cloning artifact due to thrombin cleavage

1   GLYSERLEUGLNASPHISILELYSVALTHR
2   GLNGLUGLNTYRGLULEUTYRCYSGLUMET
3   GLYSERTHRPHEGLNLEUCYSLYSILECYS
4   ALAGLUASNASPLYSASPVALLYSILEGLU
5   PROCYSGLYHISLEUMETCYSTHRSERCYS
6   LEUTHRSERTRPGLNGLUSERGLUGLYGLN
7   GLYCYSPROPHECYSARGCYSGLUILELYS
8   GLYTHRGLUPROILEVALVALASPPROPHE
9   ASPPROARG

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1, [U-99% 15N], 1 ± 0.2 mM; potassium phosphate 50 mM; sodium chloride 100 mM; zinc chloride 100 uM; PMSF 0.2 mM; DTT 5 mM

sample_2: entity_1, [U-99% 13C; U-99% 15N], 1 ± 0.2 mM; potassium phosphate 50 mM; sodium chloride 100 mM; zinc chloride 100 uM; PMSF 0.2 mM; DTT 5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HNCACOsample_2isotropicsample_conditions_1
3D CNH-NOESYsample_2isotropicsample_conditions_1

Software:

CYANA v2.1.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAC26087 BAE32253
EMBL CAA40393
GB AAF43710 AAG60692 AAH76671 AAH93450 AAI21225
REF NP_001006802 NP_001122110 NP_001245076 NP_005179 NP_031645
SP P22681 P22682 Q6DFR2
TPG DAA22315
AlphaFold P22681 P22682 Q6DFR2

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