BMRB Entry 15789

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15789

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Title:
Chemical shift assignments for human MCFD2
Deposition date:
2008-05-30
Original release date:
2008-08-28
Authors:
Guy, Jodie; Wigren, Edvard; Hard, Torleif; Lindqvist, Ylva
Citation:

Citation: Guy, Jodie; Wigren, Edvard; Svard, Maria; Hard, Torleif; Lindqvist, Ylva. "New insights into multiple coagulation factor deficiency from the solution structure of human MCFD2"  J. Mol. Biol. 381, 941-955 (2008).
PubMed: 18590741

Assembly members:

Assembly members:
MCFD2, polymer, 124 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MCFD2: GSHMEEPAASFSQPGSMGLD KNTVHDQEHIMEHLEGVINK PEAEMSPQELQLHYFKMHDY DGNNLLDGLELSTAITHVHK EEGSEQAPLMSEDELINIID GVLRDDDKNNDGYIDYAEFA KSLQ

Data sets:
Data typeCount
13C chemical shifts703
15N chemical shifts108
1H chemical shifts1174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain A1
2Calcium ion 12
3Calcium ion 22

Entities:

Entity 1, Chain A 124 residues - Formula weight is not available

1   GLYSERHISMETGLUGLUPROALAALASER
2   PHESERGLNPROGLYSERMETGLYLEUASP
3   LYSASNTHRVALHISASPGLNGLUHISILE
4   METGLUHISLEUGLUGLYVALILEASNLYS
5   PROGLUALAGLUMETSERPROGLNGLULEU
6   GLNLEUHISTYRPHELYSMETHISASPTYR
7   ASPGLYASNASNLEULEUASPGLYLEUGLU
8   LEUSERTHRALAILETHRHISVALHISLYS
9   GLUGLUGLYSERGLUGLNALAPROLEUMET
10   SERGLUASPGLULEUILEASNILEILEASP
11   GLYVALLEUARGASPASPASPLYSASNASN
12   ASPGLYTYRILEASPTYRALAGLUPHEALA
13   LYSSERLEUGLN

Entity 2, Calcium ion 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: MCFD2, [U-99% 15N], 0.5 mM; Calcium 30 mM

sample_2: MCFD2, [U-99% 13C; U-99% 15N], 0.5 mM; Calcium 30 mM

sample_3: MCFD2, [U-99% 13C; U-99% 15N], 0.5 mM; Calcium 30 mM

sample_condition_H2O_D2O_samples: ionic strength: 0.03 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_condition_D2O_samples: ionic strength: 0.03 M; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_condition_H2O_D2O_samples
2D 1H-13C HSQCsample_2isotropicsample_condition_H2O_D2O_samples
3D CBCA(CO)NHsample_2isotropicsample_condition_H2O_D2O_samples
3D CBCANHsample_2isotropicsample_condition_H2O_D2O_samples
3D HNCOsample_2isotropicsample_condition_H2O_D2O_samples
3D HCCH-COSYsample_2isotropicsample_condition_H2O_D2O_samples
3D 1H-15N TOCSYsample_1isotropicsample_condition_H2O_D2O_samples
3D 1H-15N NOESYsample_1isotropicsample_condition_H2O_D2O_samples
(HB)CB(CGCDCE)HEsample_2isotropicsample_condition_H2O_D2O_samples
3D 13C-edited NOESYsample_2isotropicsample_condition_H2O_D2O_samples
3D 13C-edited NOESYsample_3isotropicsample_condition_D2O_samples

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ANALYSIS, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB
DBJ BAF84816
EMBL CAD38756 CAH18359 CAH90418 CAH91759 CAH91764
GB AAH37845 AAH40357 AAM28465 AAP23162 AAY15013
REF NP_001127284 NP_001164977 NP_001164978 NP_001164979 NP_001164980
SP Q5R8Z6 Q8NI22
AlphaFold Q5R8Z6 Q8NI22

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks