BMRB Entry 15779

Name: Backbone 1H, 13C and 15N assignments for ginkbilobin-2 with C-terminal His6-tag
Published: 2009-10-14

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15779

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N assignments for ginkbilobin-2 with C-terminal His6-tag

Deposition date:

2008-05-20

Original release date:

2009-10-14

Authors:

Miyakawa, Takuya; Sawano, Yoriko; Hatano, Ken-ichi; Tanokura, Masaru

Citation:

Citation: Miyakawa, Takuya; Miyazono, Ken-ichi; Sawano, Yoriko; Hatano, Ken-ichi; Tanokura, Masaru. "Crystal structure of ginkbilobin-2 with homology to the extracellular domain of plant cysteine-rich receptor-like kinases" Proteins 77, 247-251 (2009).
PubMed: 19603485

Assembly members:

Assembly members:
ginkbilobin-2, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: ginkgo Taxonomy ID: 3311 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Ginkgo biloba

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET26b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ginkbilobin-2: MANTAFVSSACNTQKIPSGS PFNRNLRAMLADLRQNTAFS GYDYKTSRAGSGGAPTAYGR ATCKQSISQSDCTACLSNLV NRIFSICNNAIGARVQLVDC FIQYEQRSFLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	390
15N chemical shifts	120
1H chemical shifts	135

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ginkbilobin-2	1

Entities:

Entity 1, ginkbilobin-2 117 residues - Formula weight is not available

1

MET

ALA

ASN

THR

ALA

PHE

VAL

SER

ALA

2

CYS

ASN

THR

GLN

LYS

ILE

PRO

SER

GLY

SER

3

PRO

PHE

ASN

ARG

ASN

LEU

ARG

ALA

MET

LEU

4

ALA

ASP

LEU

ARG

GLN

ASN

THR

ALA

PHE

SER

5

GLY

TYR

ASP

TYR

LYS

THR

SER

ARG

ALA

GLY

6

SER

GLY

ALA

PRO

THR

ALA

TYR

GLY

ARG

7

ALA

THR

CYS

LYS

GLN

SER

ILE

SER

GLN

SER

8

ASP

CYS

THR

ALA

CYS

LEU

SER

ASN

LEU

VAL

9

ASN

ARG

ILE

PHE

SER

ILE

CYS

ASN

ALA

10

ILE

GLY

ALA

ARG

VAL

GLN

LEU

VAL

ASP

CYS

11

PHE

ILE

GLN

TYR

GLU

GLN

ARG

SER

PHE

LEU

12

GLU

HIS

Samples:

sample_1: ginkbilobin-2, [U-99% 13C; U-99% 15N], 0.95 mM; H2O 92%; D2O 8%

sample_conditions_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

PDB	3A2E 4XRE
GB	ABF55255 ACP27608 ACZ57929
SP	A4ZDL6
AlphaFold	A4ZDL6