BMRB Entry 15769

Title:
1H,13C, and 15N resonance assignment of the ubiquitin like domain (UBL) of Dsk2p.
Deposition date:
2008-05-12
Original release date:
2008-09-12
Authors:
Zhang, Daoning; Chen, Tony; Fushman, David
Citation:

Citation: Chen, Tony; Zhang, Daoning; Matiuhin, Yulia; Glickman, Michael; Fushman, David. "1H, 13C, and 15N resonance assignment of the ubiquitin-like domain from Dsk2p"  Biomol. NMR Assignments 2, 147-149 (2008).
PubMed: 19636891

Assembly members:

Assembly members:
Dsk2_UBL, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-30

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts82
1H chemical shifts589
residual dipolar couplings79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBL domain1

Entities:

Entity 1, UBL domain 97 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERSERLEUASNILEHISILELYSSER
3   GLYGLNASPLYSTRPGLUVALASNVALALA
4   PROGLUSERTHRVALLEUGLNPHELYSGLU
5   ALAILEASNLYSALAASNGLYILEPROVAL
6   ALAASNGLNARGLEUILETYRSERGLYLYS
7   ILELEULYSASPASPGLNTHRVALGLUSER
8   TYRHISILEGLNASPGLYHISSERVALHIS
9   LEUVALLYSSERGLNPROLYSPROLEUASP
10   LEUGLNPROSERLEUILESER

Samples:

sample_1: Dsk2 UBL, [U-100% 13C; U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium azide 0.002 w/v; H2O 93%; D2O 7%

sample_2: Dsk2 UBL, [U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium azide 0.002 w/v; H2O 93%; D2O 7%

sample_3: Dsk2 UBL, [U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium azide 0.002 w/v; liquid crystal for RDC 0.05 w/v; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 296 K

sample_conditions_2: pH: 6.8; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
HSQC_IPsample_3isotropicsample_conditions_2
HSQC_APsample_3anisotropicsample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ GAA25728
EMBL CAA89774 CAY82109
GB AAB07267 AHY76732 AJP40971 AJS62143 AJS62578
REF NP_014003
SP P48510
TPG DAA10177
AlphaFold P48510

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks