BMRB Entry 15769

Name: 1H,13C, and 15N resonance assignment of the ubiquitin like domain (UBL) of Dsk2p.
Published: 2008-09-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15769

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,13C, and 15N resonance assignment of the ubiquitin like domain (UBL) of Dsk2p.

Deposition date:

2008-05-12

Original release date:

2008-09-12

Authors:

Zhang, Daoning; Chen, Tony; Fushman, David

Citation:

Citation: Chen, Tony; Zhang, Daoning; Matiuhin, Yulia; Glickman, Michael; Fushman, David. "1H, 13C, and 15N resonance assignment of the ubiquitin-like domain from Dsk2p" Biomol. NMR Assignments 2, 147-149 (2008).
PubMed: 19636891

Assembly members:

Assembly members:
Dsk2_UBL, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dsk2_UBL: MRGSHHHHHHGSSLNIHIKS GQDKWEVNVAPESTVLQFKE AINKANGIPVANQRLIYSGK ILKDDQTVESYHIQDGHSVH LVKSQPKPLDLQPSLIS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
RDCs
- RDC

Data type	Count
13C chemical shifts	357
15N chemical shifts	82
1H chemical shifts	589
residual dipolar couplings	79

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBL domain	1

Entities:

Entity 1, UBL domain 97 residues - Formula weight is not available

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

LEU

ASN

ILE

HIS

ILE

LYS

SER

3

GLY

GLN

ASP

LYS

TRP

GLU

VAL

ASN

VAL

ALA

4

PRO

GLU

SER

THR

VAL

LEU

GLN

PHE

LYS

GLU

5

ALA

ILE

ASN

LYS

ALA

ASN

GLY

ILE

PRO

VAL

6

ALA

ASN

GLN

ARG

LEU

ILE

TYR

SER

GLY

LYS

7

ILE

LEU

LYS

ASP

GLN

THR

VAL

GLU

SER

8

TYR

HIS

ILE

GLN

ASP

GLY

HIS

SER

VAL

HIS

9

LEU

VAL

LYS

SER

GLN

PRO

LYS

PRO

LEU

ASP

10

LEU

GLN

PRO

SER

LEU

ILE

SER

Samples:

sample_1: Dsk2 UBL, [U-100% 13C; U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium azide 0.002 w/v; H2O 93%; D2O 7%

sample_2: Dsk2 UBL, [U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium azide 0.002 w/v; H2O 93%; D2O 7%

sample_3: Dsk2 UBL, [U-100% 15N], 2 mM; sodium phosphate 20 mM; sodium azide 0.002 w/v; liquid crystal for RDC 0.05 w/v; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 296 K

sample_conditions_2: pH: 6.8; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
HSQC_IP	sample_3	isotropic	sample_conditions_2
HSQC_AP	sample_3	anisotropic	sample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2BWE 2BWF
DBJ	GAA25728
EMBL	CAA89774 CAY82109
GB	AAB07267 AHY76732 AJP40971 AJS62143 AJS62578
REF	NP_014003
SP	P48510
TPG	DAA10177
AlphaFold	P48510