BMRB Entry 15764

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Ca2+-Binding Domain 1 of the Na+/Ca2+ Exchanger in the Absence of Ca2+
Published: 2008-06-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15764

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Ca2+-Binding Domain 1 of the Na+/Ca2+ Exchanger in the Absence of Ca2+

Deposition date:

2008-05-09

Original release date:

2008-06-30

Authors:

Johnson, Eric; Bruschweiler-Li, Lei; Showalter, Scott; Vuister, Geerten; Zhang, Fengli; Bruschweiler, Rafael

Citation:

Citation: Johnson, Eric; Bruschweiler-Li, Lei; Showalter, Scott; Vuister, Geerten; Zhang, Fengli; Bruschweiler, Rafael. "Structure and Dynamics of Ca2+-Binding Domain 1 of the Na+/Ca2+ Exchanger in the Presence and in the Absence of Ca2+" J. Mol. Biol. 377, 945-955 (2008).
PubMed: 18280495

Assembly members:

Assembly members:
CBD1, polymer, 147 residues, 16193 Da.

Natural source:

Natural source: Common Name: Dog Taxonomy ID: 9615 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Canis familiaris

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBD1: MSHHHHHHVSKIFFEQGTYQ CLENCGTVALTIIRRGGDLT NTVFVDFRTEDGTANAGSDY EFTEGTVVFKPGETQKEIRV GIIDDDIFEEDENFLVHLSN VKVSSEASEDGILEANHVSA LACLGSPSTATVTIFDDDHA GIFTFEE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	356
15N chemical shifts	127
1H chemical shifts	127

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CBD1	1

Entities:

Entity 1, CBD1 147 residues - 16193 Da.

Residues 1-8 represent a non-native affinity tag.

1

MET

SER

HIS

VAL

SER

2

LYS

ILE

PHE

GLU

GLN

GLY

THR

TYR

GLN

3

CYS

LEU

GLU

ASN

CYS

GLY

THR

VAL

ALA

LEU

4

THR

ILE

ARG

GLY

ASP

LEU

THR

5

ASN

THR

VAL

PHE

VAL

ASP

PHE

ARG

THR

GLU

6

ASP

GLY

THR

ALA

ASN

ALA

GLY

SER

ASP

TYR

7

GLU

PHE

THR

GLU

GLY

THR

VAL

PHE

LYS

8

PRO

GLY

GLU

THR

GLN

LYS

GLU

ILE

ARG

VAL

9

GLY

ILE

ASP

ILE

PHE

GLU

10

ASP

GLU

ASN

PHE

LEU

VAL

HIS

LEU

SER

ASN

11

VAL

LYS

VAL

SER

GLU

ALA

SER

GLU

ASP

12

GLY

ILE

LEU

GLU

ALA

ASN

HIS

VAL

SER

ALA

13

LEU

ALA

CYS

LEU

GLY

SER

PRO

SER

THR

ALA

14

THR

VAL

THR

ILE

PHE

ASP

HIS

ALA

15

GLY

ILE

PHE

THR

PHE

GLU

Samples:

sample_1: CBD1, [U-100% 13C; U-100% 15N], 0.5 mM; HEPES 20 mM; beta-mercaptoethanol 20 mM; sodium azide 0.02%; EDTA 15 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 306 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2DPK 2FWS
DBJ	BAF84385
EMBL	CAH91585
GB	AAA97928 AAB40148 AAD04173 AAD04174 AAD17213
REF	NP_001028033 NP_001106272 NP_001106273 NP_001125933 NP_001164429