BMRB Entry 15759

Name: 1H, 13C and 15N backbone chemical shift assignments of the 3FNIII module from human fibronectin
Published: 2009-04-15

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15759

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N backbone chemical shift assignments of the 3FNIII module from human fibronectin

Deposition date:

2008-05-06

Original release date:

2009-04-15

Authors:

Vakonakis, Ioannis; Campbell, Iain

Citation:

Citation: Vakonakis, Ioannis; Staunton, David; Ellis, Ian; Sarkies, Peter; Flanagan, Aleksandra; Schor, Ana; Schor, Seth; Campbell, Iain. "Motogenic sites in human fibronectin are masked by long range interactions" J. Biol. Chem. 284, 15668-15675 (2009).
PubMed: 19366708

Assembly members:

Assembly members:
3F3, polymer, 96 residues, 10256.2 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
3F3: GPLGSAPDAPPDPTVDQVDD TSIVVRWSRPQAPITGYRIV YSPSVEGSSTELNLPETANS VTLSDLQPGVQYNITIYAVE ENQESTPVVIQQETTG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	178
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein	1

Entities:

Entity 1, protein 96 residues - 10256.2 Da.

1

GLY

PRO

LEU

GLY

SER

ALA

PRO

ASP

ALA

PRO

2

PRO

ASP

PRO

THR

VAL

ASP

GLN

VAL

ASP

3

THR

SER

ILE

VAL

ARG

TRP

SER

ARG

PRO

4

GLN

ALA

PRO

ILE

THR

GLY

TYR

ARG

ILE

VAL

5

TYR

SER

PRO

SER

VAL

GLU

GLY

SER

THR

6

GLU

LEU

ASN

LEU

PRO

GLU

THR

ALA

ASN

SER

7

VAL

THR

LEU

SER

ASP

LEU

GLN

PRO

GLY

VAL

8

GLN

TYR

ASN

ILE

THR

ILE

TYR

ALA

VAL

GLU

9

GLU

ASN

GLN

GLU

SER

THR

PRO

VAL

ILE

10

GLN

GLU

THR

GLY

Samples:

sample_1: 3F3 0.7 mM; D2O 5%; DSS 0.1 mM; sodium chloride 20 mM; sodium phosphate 20 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 115 mM; pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP v4.3.7, Garrett - data analysis

TOPSPIN v1.3, Bruker Biospin - collection

NMR spectrometers:

Bruker DMX 500 MHz

BMRB	25564
PDB	2N1K