BMRB Entry 15758

Name: 1H, 13C and 15N backbone chemical shift assignments of the 4FNI-5FNI module pair from human fibronectin
Published: 2009-04-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15758

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N backbone chemical shift assignments of the 4FNI-5FNI module pair from human fibronectin

Deposition date:

2008-05-06

Original release date:

2009-04-15

Authors:

Vakonakis, Ioannis; Campbell, Iain

Citation:

Citation: Vakonakis, Ioannis; Staunton, David; Ellis, Ian; Sarkies, Peter; Flanagan, Aleksandra; Schor, Ana; Schor, Seth; Campbell, Iain. "Motogenic sites in human fibronectin are masked by long range interactions" J. Biol. Chem. 284, 15668-15675 (2009).
PubMed: 19366708

Assembly members:

Assembly members:
4F15F1, polymer, 93 residues, 10519.6 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPIC9K

Entity Sequences (FASTA):

Entity Sequences (FASTA):
4F15F1: AEKCFDHAAGTSYVVGETWE KPYQGWMMVDCTCLGEGSGR ITCTSRNRCNDQDTRTSYRI GDTWSKKDNRGNLLQCICTG NGRGEWKCERHTS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	175
15N chemical shifts	96
1H chemical shifts	98

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein	1

Entities:

Entity 1, protein 93 residues - 10519.6 Da.

1

ALA

GLU

LYS

CYS

PHE

ASP

HIS

ALA

GLY

2

THR

SER

TYR

VAL

GLY

GLU

THR

TRP

GLU

3

LYS

PRO

TYR

GLN

GLY

TRP

MET

VAL

ASP

4

CYS

THR

CYS

LEU

GLY

GLU

GLY

SER

GLY

ARG

5

ILE

THR

CYS

THR

SER

ARG

ASN

ARG

CYS

ASN

6

ASP

GLN

ASP

THR

ARG

THR

SER

TYR

ARG

ILE

7

GLY

ASP

THR

TRP

SER

LYS

ASP

ASN

ARG

8

GLY

ASN

LEU

GLN

CYS

ILE

CYS

THR

GLY

9

ASN

GLY

ARG

GLY

GLU

TRP

LYS

CYS

GLU

ARG

10

HIS

THR

SER

Samples:

sample_1: 4F15F1, [U-98% 13C; U-98% 15N], 0.2 mM; D2O 5%; sodium azide 0.01%; sodium chloride 20 mM; sodium phosphate 20 mM; DSS 0.1 mM

sample_conditions_1: ionic strength: 115 mM; pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP v4.3.7, Garrett - data analysis

TOPSPIN v1.3, Bruker Biospin - collection

NMR spectrometers:

Bruker DMX 500 MHz