BMRB Entry 15747

Name: Structural and Functional Characterization of TM IX of the NHE1 Isoform of the Na+/H+ Exchanger
Published: 2008-05-29

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PDB ID: 2k3c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15747
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural and Functional Characterization of TM IX of the NHE1 Isoform of the Na+/H+ Exchanger

Deposition date:

2008-05-01

Original release date:

2008-05-29

Authors:

Reddy, Tyler; Ding, J.; Li, X.; Sykes, Brian; Fliegel, Larry; Rainey, Jan

Citation:

Citation: Reddy, Tyler; Ding, J.; Li, X.; Sykes, Brian; Rainey, Jan; Fliegel, Larry. "Structural and Functional Characterization of TM IX of the NHE1 Isoform of the Na+/H+ Exchanger" J. Biol. Chem. 283, 22018-22030 (2008).
PubMed: 18508767

Assembly members:

Assembly members:
TMIX_peptide, polymer, 33 residues, 3444.226 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TMIX_peptide: XKSYMAYLSAELFHLSGIMA LIASGVVMRPKKX

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	235
13C chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TMIX_peptide	1

Entities:

Entity 1, TMIX_peptide 33 residues - 3444.226 Da.

1

ACE

LYS

SER

TYR

MET

ALA

TYR

LEU

SER

ALA

2

GLU

LEU

PHE

HIS

LEU

SER

GLY

ILE

MET

ALA

3

LEU

ILE

ALA

SER

GLY

VAL

MET

ARG

PRO

4

LYS

NH2

Samples:

sample_TMIX_peptide: TMIX peptide 0.9 ± 0.1 mM; DPC-d38 75 mM; DSS 0.25 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 303.15 K; pH: 5.05; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_TMIX_peptide	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_TMIX_peptide	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_TMIX_peptide	isotropic	sample_conditions_1

Software:

X-PLOR_NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY v3.112, Goddard - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2K3C
DBJ	BAE29332 BAE32882 BAE38221 BAG36869 BAG62158
EMBL	CAA42558 CAA43721 CAA48771 CAH23227
GB	AAA31092 AAA91483 AAA92976 AAA98479 AAB20633
REF	NP_001007104 NP_001095191 NP_001233682 NP_001273957 NP_003038
SP	P19634 P23791 P26431 P48761 P48762
TPG	DAA32066
AlphaFold	P19634 P23791 P26431 P48761 P48762