BMRB Entry 15742
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15742
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Pedersen, Marie; Underhaug, Jarl; Dittmer, Jens; Miller, Mette; Nielsen, Niels. "The three-dimensional structure of CsmA: A small antenna protein from the green sulfur bacterium Chlorobium tepidum" FEBS Lett. 582, 2869-2874 (2008).
PubMed: 18652828
Assembly members:
CsmA, polymer, 59 residues, 6160.066 Da.
Natural source: Common Name: Chlorobium tepidum Taxonomy ID: 1097 Superkingdom: Bacteria Kingdom: not available Genus/species: Chlorobium tepidum
Experimental source: Production method: purified from the natural source Host organism: Chlorobium tepidum
Entity Sequences (FASTA):
CsmA: MSGGGVFTDILAAAGRIFEV
MVEGHWETVGMLFDSLGKGT
MRINRNAYGSMGGGSLRGS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 369 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CsmA | 1 |
Entities:
Entity 1, CsmA 59 residues - 6160.066 Da.
| 1 | MET | SER | GLY | GLY | GLY | VAL | PHE | THR | ASP | ILE | ||||
| 2 | LEU | ALA | ALA | ALA | GLY | ARG | ILE | PHE | GLU | VAL | ||||
| 3 | MET | VAL | GLU | GLY | HIS | TRP | GLU | THR | VAL | GLY | ||||
| 4 | MET | LEU | PHE | ASP | SER | LEU | GLY | LYS | GLY | THR | ||||
| 5 | MET | ARG | ILE | ASN | ARG | ASN | ALA | TYR | GLY | SER | ||||
| 6 | MET | GLY | GLY | GLY | SER | LEU | ARG | GLY | SER |
Samples:
sample_1: CsmA, [U-100% 15N], 1.4 mM
sample_conditions_1: ionic strength: 0 M; pH: .; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D NOESY-HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D TOCSY-HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
TOPSPIN, Bruker - collection
ARIA v2.2, Linge, O'Donoghue and Nilges - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 700 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks