BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15741

Title: 1H, 15N and 13C resonance assignment of imidazole glycerol phosphate (IGP) synthase protein HisF from Thermotoga maritima   PubMed: 19636909

Deposition date: 2008-04-22 Original release date: 2008-10-14

Authors: Lipchock, James; Loria, J.

Citation: Lipchock, James; Loria, J. Patrick. "1H, 15N and 13C resonance assignment of imidazole glycerol phosphate (IGP) synthase protein HisF from Thermotoga maritima"  Biomol. NMR Assignments 2, 219-221 (2008).

Assembly members:
HisF, polymer, 253 residues, 27718 Da.

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: TM hisF pET14b

Entity Sequences (FASTA):
HisF: MLAKRIIACLDVKDGRVVKG TNFENLRDSGDPVELGKFYS EIGIDELVFLDITASVEKRK TMLELVEKVAEQIDIPFTVG GGIHDFETASELILRGADKV SINTAAVENPSLITQIAQTF GSQAVVVAIDAKRVDGEFMV FTYSGKKNTGILLRDWVVEV EKRGAGEILLTSIDRDGTKS GYDTEMIRFVRPLTTLPIIA SGGAGKMEHFLEAFLAGADA ALAASVFHFREIDVRELKEY LKKHGVNVRLEGL

Data sets:
Data typeCount
13C chemical shifts720
15N chemical shifts239
1H chemical shifts239

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TM hisF1

Entities:

Entity 1, TM hisF 253 residues - 27718 Da.

Thermotoga maritima Imidazole Glycerol Phosphate (IGP) Synthase HisF

1   METLEUALALYSARGILEILEALACYSLEU
2   ASPVALLYSASPGLYARGVALVALLYSGLY
3   THRASNPHEGLUASNLEUARGASPSERGLY
4   ASPPROVALGLULEUGLYLYSPHETYRSER
5   GLUILEGLYILEASPGLULEUVALPHELEU
6   ASPILETHRALASERVALGLULYSARGLYS
7   THRMETLEUGLULEUVALGLULYSVALALA
8   GLUGLNILEASPILEPROPHETHRVALGLY
9   GLYGLYILEHISASPPHEGLUTHRALASER
10   GLULEUILELEUARGGLYALAASPLYSVAL
11   SERILEASNTHRALAALAVALGLUASNPRO
12   SERLEUILETHRGLNILEALAGLNTHRPHE
13   GLYSERGLNALAVALVALVALALAILEASP
14   ALALYSARGVALASPGLYGLUPHEMETVAL
15   PHETHRTYRSERGLYLYSLYSASNTHRGLY
16   ILELEULEUARGASPTRPVALVALGLUVAL
17   GLULYSARGGLYALAGLYGLUILELEULEU
18   THRSERILEASPARGASPGLYTHRLYSSER
19   GLYTYRASPTHRGLUMETILEARGPHEVAL
20   ARGPROLEUTHRTHRLEUPROILEILEALA
21   SERGLYGLYALAGLYLYSMETGLUHISPHE
22   LEUGLUALAPHELEUALAGLYALAASPALA
23   ALALEUALAALASERVALPHEHISPHEARG
24   GLUILEASPVALARGGLULEULYSGLUTYR
25   LEULYSLYSHISGLYVALASNVALARGLEU
26   GLUGLYLEU

Samples:

sample_TM_hisF: HisF, [U-100% 13C; U-100% 15N; 80% 2H], 0.45 mM; MES 10 mM; potassium chloride 50 mM; EDTA 1 mM

sample_conditions_TM_hisF: ionic strength: 0.11 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_TM_hisFisotropicsample_conditions_TM_hisF
3D HNCOsample_TM_hisFisotropicsample_conditions_TM_hisF
3D HNCAsample_TM_hisFisotropicsample_conditions_TM_hisF
3D HN(CA)CBsample_TM_hisFisotropicsample_conditions_TM_hisF
3D HN(COCA)CBsample_TM_hisFisotropicsample_conditions_TM_hisF
3D HN(CO)CAsample_TM_hisFisotropicsample_conditions_TM_hisF
3D HN(CA)COsample_TM_hisFisotropicsample_conditions_TM_hisF

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
GB AAD36113 ABQ47719 ACB10103 ADA67795 AGL49964
REF NP_228842 WP_004080486 WP_008193378 WP_011944125 WP_012311332
SP A5INE6 B1L873 Q9X0C6

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts