BMRB Entry 15738

Name: Carbon storage regulator from Helicobacter pylori
Published: 2014-03-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15738

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Carbon storage regulator from Helicobacter pylori

Deposition date:

2008-04-21

Original release date:

2014-03-05

Authors:

Yang, Chun-Jun; Chen, Heng-Li; Cheng, Hsi-Tsung; Chiu, Chui-Lin; Cheng, Jya-Wei

Citation:

Citation: Yang, Chun-Jun; Chen, Heng-Li; Cheng, Hsi-Tsung; Chiu, Chui-Lin; Cheng, Jya-Wei. "Structural Characterization and DNA Binding Sequence Selection of the CsrA Protein from Helicobacter pylori" .

Assembly members:

Assembly members:
HP1442, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETBlue-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HP1442: MALILSRKVNEGIVIDDNIH IKVISIDRGSVRLGFEAPES TLILRAELKEAIVSENQKAS VCVDESLLENIKKVIKPLEH HHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	75
1H chemical shifts	147

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HP1442, chain A	1
2	HP1442, chain B	1

Entities:

Entity 1, HP1442, chain A 85 residues - Formula weight is not available

Residues 78-85 represent a non-native affinity tag and residue 2 is an artefact from cloning.

1

MET

ALA

LEU

ILE

LEU

SER

ARG

LYS

VAL

ASN

2

GLU

GLY

ILE

VAL

ILE

ASP

ASN

ILE

HIS

3

ILE

LYS

VAL

ILE

SER

ILE

ASP

ARG

GLY

SER

4

VAL

ARG

LEU

GLY

PHE

GLU

ALA

PRO

GLU

SER

5

THR

LEU

ILE

LEU

ARG

ALA

GLU

LEU

LYS

GLU

6

ALA

ILE

VAL

SER

GLU

ASN

GLN

LYS

ALA

SER

7

VAL

CYS

VAL

ASP

GLU

SER

LEU

GLU

ASN

8

ILE

LYS

VAL

ILE

LYS

PRO

LEU

GLU

HIS

9

HIS

Samples:

sample_1: HP1442, [U-13C; U-15N], 1 mM; potassium phosphate 30 mM; sodium chloride 50 mM; EDTA 0.1 mM; DTT 5 mM; sodium azide 10 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

xwinnmr, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

GB	NP_208233 AAD08480 ABF85435 ACD48869 ACJ08568 ACX98483
UNP	O25983
DBJ	BAJ57406 BAJ58911 BAJ60431 BAM97263 BAM98719
EMBL	CAX30116 CBI65651
REF	NP_208233 WP_000906438 WP_000906439 WP_000906441 WP_000906442
SP	B2UVI7 B6JNU0 O25983
AlphaFold	O25983 B2UVI7 B6JNU0 O25983