BMRB Entry 15733

Name: Solution structure of Lamin B Receptor Tudor domain
Published: 2012-07-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15733

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Lamin B Receptor Tudor domain

Deposition date:

2008-04-16

Original release date:

2012-07-19

Authors:

Liokatis, S.; Edlich, C.; Georgatos, SD.; Sattler, M.; Politou, AS.

Citation:

Citation: Liokatis, Stamatis; Edlich, Christian; Soupsana, Katerina; Giannios, Ioannis; Panagiotidou, Parthena; Tripsianes, Konstantinos; Sattler, Michael; Georgatos, Spyros; Politou, Anastasia. "Solution structure and molecular interactions of lamin B receptor Tudor domain" J. Biol. Chem. 287, 1032-1042 (2012).
PubMed: 22052904

Assembly members:

Assembly members:
chicken_Lamin_B_Receptor, polymer, 66 residues, 7490.461 Da.

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
chicken_Lamin_B_Receptor: GAMGMPNRKYADGEVVMGRW PGSVLYYEVQVTSYDDASHL YTVKYKDGTELALKESDIRL QSSFKQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	230
15N chemical shifts	69
1H chemical shifts	452

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	chicken Lamin B Receptor	1

Entities:

Entity 1, chicken Lamin B Receptor 66 residues - 7490.461 Da.

1

GLY

ALA

MET

GLY

MET

PRO

ASN

ARG

LYS

TYR

2

ALA

ASP

GLY

GLU

VAL

MET

GLY

ARG

TRP

3

PRO

GLY

SER

VAL

LEU

TYR

GLU

VAL

GLN

4

VAL

THR

SER

TYR

ASP

ALA

SER

HIS

LEU

5

TYR

THR

VAL

LYS

TYR

LYS

ASP

GLY

THR

GLU

6

LEU

ALA

LEU

LYS

GLU

SER

ASP

ILE

ARG

LEU

7

GLN

SER

PHE

LYS

GLN

Samples:

sample_1: LBR Tudor, [U-13C; U-15N], 0.5-1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O 10%; H2O 90%

sample_2: LBR Tudor, [U-13C; U-15N], 0.5-1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-separated_NOESY	sample_1	isotropic	sample_conditions_1
3D_13C-separated_NOESY	sample_2	isotropic	sample_conditions_1
3D_13C-separated_NOESY-aromatic region	sample_2	isotropic	sample_conditions_1
2D-13C-HSQC	sample_2	isotropic	sample_conditions_1
2D-15N-HSQC	sample_1	isotropic	sample_conditions_1
HNCA	sample_1	isotropic	sample_conditions_1
CBCANH	sample_1	isotropic	sample_conditions_1
CBCA-(CO)NH	sample_1	isotropic	sample_conditions_1
HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D-CBHD	sample_2	isotropic	sample_conditions_1
2D-CBHE	sample_2	isotropic	sample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz

BMRB	17402
PDB	2L8D
EMBL	CAA68758 CAG31779
REF	NP_990673 XP_010704965
SP	P23913
AlphaFold	P23913