BMRB Entry 15729

Title:
Solution Structure of Tick Carboxypeptidase Inhibitor at pH 3.5
Deposition date:
2008-04-15
Original release date:
2008-07-15
Authors:
Pantoja-Uceda, David; Blanco, Francsico
Citation:

Citation: Pantoja-Uceda, David; Arolas, Joan L.; Garcia, Pascal; Lopez-Hernandez, Eva; Padro, Daniel; Aviles, Francesc X.; Blanco, Francisco J.. "The NMR structure and dynamics of the two-domain tick carboxypeptidase inhibitor reveal flexibility in its free form and stiffness upon binding to human carboxypeptidase B"  Biochemistry 47, 7066-7078 (2008).
PubMed: 18558717

Assembly members:

Assembly members:
TCI, polymer, 75 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 67831   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rhipicephalus bursa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBAT-4-OmpA

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TCI1

Entities:

Entity 1, TCI 75 residues - Formula weight is not available

1   ASNGLUCYSVALSERLYSGLYPHEGLYCYS
2   LEUPROGLNSERASPCYSPROGLNGLUALA
3   ARGLEUSERTYRGLYGLYCYSSERTHRVAL
4   CYSCYSASPLEUSERLYSLEUTHRGLYCYS
5   LYSGLYLYSGLYGLYGLUCYSASNPROLEU
6   ASPARGGLNCYSLYSGLULEUGLNALAGLU
7   SERALASERCYSGLYLYSGLYGLNLYSCYS
8   CYSVALTRPLEUHIS

Samples:

sample_1: TCI 1.00 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: . M; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRView v5.0.4, Johnson, One Moon Scientific - data analysis, peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

xwinnmr, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 15372 15730 15731
PDB
GB AAW72225
SP Q5EPH2
AlphaFold Q5EPH2