BMRB Entry 15727

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15727

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Title:
Full 1H, 13C, and 15N Chemical Shift Assignments for La NTD complexed with 5'UUUU RNA
Deposition date:
2008-04-14
Original release date:
2008-11-11
Authors:
Conte, Maria; Sanfelice, Domenico; Kelly, Geoff; Curry, Stephen
Citation:

Citation: Sanfelice, Domenico; Kelly, Geoff; Curry, Stephen; Conte, Maria. "NMR assignment of the N-terminal region of human La free and in complex with RNA"  Biomol. NMR Assignments 2, 107-109 (2008).
PubMed: 19636881

Assembly members:

Assembly members:
La_protein, polymer, 197 residues, Formula weight is not available
UUUU_RNA, polymer, 4 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
La_protein: MAENGDNEKMAALEAKICHQ IEYYFGDFNLPRDKFLKEQI KLDEGWVPLEIMIKFNRLNR LTTDFNVIVEALSKSKAELM EISEDKTKIRRSPSKPLPEV TDEYKNDVKNRSVYIKGFPT DATLDDIKEWLEDKGQVLNI QMRRTLHKAFKGSIFVVFDS IESAKKFVETPGQKYKETDL LILFKDDYFAKKNEVDS
UUUU_RNA: UUUU

Data sets:
Data typeCount
13C chemical shifts768
15N chemical shifts180
1H chemical shifts1118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1La NTD1
25'UUUU RNA2

Entities:

Entity 1, La NTD 197 residues - Formula weight is not available

1   METALAGLUASNGLYASPASNGLULYSMET
2   ALAALALEUGLUALALYSILECYSHISGLN
3   ILEGLUTYRTYRPHEGLYASPPHEASNLEU
4   PROARGASPLYSPHELEULYSGLUGLNILE
5   LYSLEUASPGLUGLYTRPVALPROLEUGLU
6   ILEMETILELYSPHEASNARGLEUASNARG
7   LEUTHRTHRASPPHEASNVALILEVALGLU
8   ALALEUSERLYSSERLYSALAGLULEUMET
9   GLUILESERGLUASPLYSTHRLYSILEARG
10   ARGSERPROSERLYSPROLEUPROGLUVAL
11   THRASPGLUTYRLYSASNASPVALLYSASN
12   ARGSERVALTYRILELYSGLYPHEPROTHR
13   ASPALATHRLEUASPASPILELYSGLUTRP
14   LEUGLUASPLYSGLYGLNVALLEUASNILE
15   GLNMETARGARGTHRLEUHISLYSALAPHE
16   LYSGLYSERILEPHEVALVALPHEASPSER
17   ILEGLUSERALALYSLYSPHEVALGLUTHR
18   PROGLYGLNLYSTYRLYSGLUTHRASPLEU
19   LEUILELEUPHELYSASPASPTYRPHEALA
20   LYSLYSASNGLUVALASPSER

Entity 2, 5'UUUU RNA 4 residues - Formula weight is not available

1   UUUU

Samples:

sample_1: La protein, [U-100% 13C; U-100% 15N], 0.3-0.4 mM; 5'UUUU RNA 0.3-0.4 mM; Tris-HCl 20 mM; KCl 100 mM; DTT 1 mM

sample_conditions_1: ionic strength: 120 mM; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

TOPSPIN, Bruker Biospin - collection

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 15726 17878
PDB
DBJ BAE87871 BAE87890 BAG70042 BAG70165 BAI45822
EMBL CAA31985 CAA31986
GB AAA36577 AAA51885 AAH01289 AAH20818 AAI03235
REF NP_001267347 NP_001270975 NP_001281074 NP_001296114 NP_003133
SP P05455 P10881
TPG DAA32804
AlphaFold P05455 P10881

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks