BMRB Entry 15716

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15716
MolProbity Validation Chart

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Title:
1H, 15N, 13C resonance assignment of the AlgE6R1 subunit from the Azotobacter vinelandii Mannuronan C5-epimerase
Deposition date:
2008-04-03
Original release date:
2008-08-08
Authors:
Aachmann, Finn
Citation:

Citation: Aachmann, Finn; Skjak-Braek, Gudmund. "1H, 15N, 13C resonance assignment of the AlgE6R1 subunit from the Azotobacter vinelandii mannuronan C5-epimerase"  Biomol. NMR Assignments 2, 123-125 (2008).
PubMed: 19636885

Assembly members:

Assembly members:
AlgE6R1, polymer, 153 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Azotobacter vinelandii   Taxonomy ID: 354   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Azotobacter vinelandii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFA8

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AlgE6R1: AQGTDGNDVLIGSDVGEQIS GGAGDDRLDGGAGDDLLDGG AGRDRLTGGLGADTFRFALR EDSHRSPLGTFSDLILDFDP SQDKIDVSALGFIGLGNGYA GTLAVSLSADGLRTYLKSYD ADAQGRSFELALDGNHAATL SAGNIVFAAATPG

Data sets:
Data typeCount
13C chemical shifts542
15N chemical shifts155
1H chemical shifts898

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AlgE6R11
2Calcium ion2

Entities:

Entity 1, AlgE6R1 153 residues - Formula weight is not available

1   ALAGLNGLYTHRASPGLYASNASPVALLEU
2   ILEGLYSERASPVALGLYGLUGLNILESER
3   GLYGLYALAGLYASPASPARGLEUASPGLY
4   GLYALAGLYASPASPLEULEUASPGLYGLY
5   ALAGLYARGASPARGLEUTHRGLYGLYLEU
6   GLYALAASPTHRPHEARGPHEALALEUARG
7   GLUASPSERHISARGSERPROLEUGLYTHR
8   PHESERASPLEUILELEUASPPHEASPPRO
9   SERGLNASPLYSILEASPVALSERALALEU
10   GLYPHEILEGLYLEUGLYASNGLYTYRALA
11   GLYTHRLEUALAVALSERLEUSERALAASP
12   GLYLEUARGTHRTYRLEULYSSERTYRASP
13   ALAASPALAGLNGLYARGSERPHEGLULEU
14   ALALEUASPGLYASNHISALAALATHRLEU
15   SERALAGLYASNILEVALPHEALAALAALA
16   THRPROGLY

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

AlgE6R1_H2O: AlgE6R1, [U-98% 13C; U-98% 15N], 0.9-1.3 ± 0.1 mM; Calcium 25 ± 0.5 mM; HEPES 20 ± 0.5 mM

AlgE6R1_D2O: AlgE6R1, [U-98% 13C; U-98% 15N], 0.9-1.3 ± 0.1 mM; Calcium 25 ± 0.5 mM; HEPES 20 ± 0.5 mM

sample_conditions_1: ionic strength: 0.165 M; pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCAlgE6R1_H2Oisotropicsample_conditions_1
2D 1H-13C HSQCAlgE6R1_D2Oisotropicsample_conditions_1
3D CBCA(CO)NHAlgE6R1_H2Oisotropicsample_conditions_1
3D HNCACBAlgE6R1_H2Oisotropicsample_conditions_1
3D HNCOAlgE6R1_H2Oisotropicsample_conditions_1
3D HN(CO)CAAlgE6R1_H2Oisotropicsample_conditions_1
3D HN(CA)COAlgE6R1_H2Oisotropicsample_conditions_1
3D HNCAAlgE6R1_H2Oisotropicsample_conditions_1
3D HBHA(CO)NHAlgE6R1_H2Oisotropicsample_conditions_1
3D HCCH-COSYAlgE6R1_D2Oisotropicsample_conditions_1
3D HCCH-TOCSYAlgE6R1_D2Oisotropicsample_conditions_1
3D 1H-15N NOESYAlgE6R1_H2Oisotropicsample_conditions_1
2D 1H-1H NOESYAlgE6R1_D2Oisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks