BMRB Entry 15715

Title:
NMR Assignments of Methionine Sulfoxide Reductase B2 from Homo sapiens
Deposition date:
2008-04-03
Original release date:
2008-10-15
Authors:
Breivik, Aashild; Aachmann, Finn; Dikiy, Alexander
Citation:

Citation: Breivik, Aashild; Aachmann, Finn; Sal, Lena; Kim, Hwa-Young; Del Conte, Rebecca; Gladyshev, Vadim; Dikiy, Alexander. "1H, 15N and 13C NMR assignments of mouse methionine sulfoxide reductase B2"  Biomol. NMR Assignments 2, 199-201 (2008).
PubMed: 19636904

Assembly members:

Assembly members:
Methionine_Sulfoxide_Reductase_B2, polymer, 143 residues, Formula weight is not available
ZN, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMsrb2ds

Data sets:
Data typeCount
13C chemical shifts516
15N chemical shifts132
1H chemical shifts812

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MsrB2dS1
2Zinc ion2

Entities:

Entity 1, MsrB2dS 143 residues - Formula weight is not available

The N-terminal signal from MsrB2 was removed (1-39). Met residue was added on the N-terminal and His-tag was added C-terminal (157-166)

1   METALAASPTRPGLNLYSLYSLEUTHRPRO
2   GLUGLNPHETYRVALTHRARGGLULYSGLY
3   THRGLUALAPROPHESERGLYMETTYRLEU
4   ASNASNLYSGLUTHRGLYMETTYRHISCYS
5   VALCYSCYSASPSERPROLEUPHESERSER
6   GLULYSLYSTYRCYSSERGLYTHRGLYTRP
7   PROSERPHESERGLUALATYRGLYSERLYS
8   GLYSERASPGLUSERHISTHRGLYILELEU
9   ARGARGLEUASPTHRSERLEUGLYCYSPRO
10   ARGMETGLUVALVALCYSLYSGLNCYSGLU
11   ALAHISLEUGLYHISVALPHEPROASPGLY
12   PROLYSPROTHRGLYGLNARGPHECYSILE
13   ASNSERVALALALEULYSPHELYSPROSER
14   LYSPROALAALAALALEUGLUHISHISHIS
15   HISHISHIS

Entity 2, Zinc ion - Formula weight is not available

1   ZN

Samples:

D2O_MsrB2dS: Methionine Sulfoxide Reductase B2, [U-98% 13C; U-98% 15N], 1.1 ± 0.1 mM; Zinc ion 1.1 ± 0.1 mM; DTT 5 ± 0.1 mM; sodium phosphate 20 ± 0.5 mM; sodium chloride 25 ± 0.5 mM

H2O_MsrB2dS: Methionine Sulfoxide Reductase B2, [U-98% 13C; U-98% 15N], 1.1 ± 0.1 mM; Zinc ion 1.1 ± 0.1 mM; DTT 5 ± 0.1 mM; sodium phosphate 20 ± 0.5 mM; sodium chloride 25 ± 0.5 mM

sample_conditions_1: ionic strength: 0.06 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCH2O_MsrB2dSisotropicsample_conditions_1
2D 1H-13C HSQCD2O_MsrB2dSisotropicsample_conditions_1
3D CBCA(CO)NHH2O_MsrB2dSisotropicsample_conditions_1
3D HNCOH2O_MsrB2dSisotropicsample_conditions_1
3D HNCAH2O_MsrB2dSisotropicsample_conditions_1
3D HNCACBH2O_MsrB2dSisotropicsample_conditions_1
3D HBHA(CO)NHH2O_MsrB2dSisotropicsample_conditions_1
3D HN(CO)CAH2O_MsrB2dSisotropicsample_conditions_1
3D HCCH-TOCSYD2O_MsrB2dSisotropicsample_conditions_1
3D HCCH-COSYD2O_MsrB2dSisotropicsample_conditions_1
3D 15N HSQC NOESYD2O_MsrB2dSisotropicsample_conditions_1

Software:

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis

xwinnmr v3.5, Bruker Biospin - collection, processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAH21619 AAH98953 EDL08114 EDL78778
REF NP_001026830 NP_083895 XP_006497626
SP Q4FZX5 Q78J03
AlphaFold Q4FZX5 Q78J03

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks