BMRB Entry 15697

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15697

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mutational and Structural Analysis of Stem-loop IIId of the Hepatitis C Virus and GB Virus B Internal Ribosome Entry Sites

Deposition date:

2008-03-28

Original release date:

2009-10-13

Authors:

Thiviyanathan, Varatharasa; Kulasegaran, Raghav; Kaluarachchi, Kumaralal; Gorenstein, David

Citation:

Citation: Thiviyanathan, Varatharasa; Kulasegaran, Raghav; Kaluarachchi, Kumaralal; Rijnbrand, Rene; Gorenstein, David. "Mutational and Structural Analysis of Stem-loop IIId of the Hepatitis C Virus and GB Virus B Internal Ribosome Entry Sites" J. Virology ., .-..

Assembly members:

Assembly members:
GBV_B_IRES, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: GB virus B Taxonomy ID: 39113 Superkingdom: Viruses Kingdom: not available Genus/species: not available GB virus B

Experimental source:

Experimental source: Production method: enzymatic synthesis Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GBV_B_IRES: GGAUGGUUGGGGUUAGCCAU CC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	179

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	domain IIId	1

Entities:

Entity 1, domain IIId 22 residues - Formula weight is not available

1

G

A

U

G

U

G

2

G

U

A

G

C

A

U

3

C

Samples:

sample_1: GBV_B_IRES 1.2 mM; potassium chloride 10 mM; sodium phosphate 10 mM; EDTA 0.05 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

VNMR, Varian - chemical shift assignment

NMR spectrometers:

Varian INOVA 750 MHz
Varian INOVA 600 MHz