BMRB Entry 15684
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15684
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NMR-STAR v3 text file.
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Citation: Zhang, Fang; Guo, Chenyun; Lou, Lili; Lin, Donghai. "Backbone and side-chain 1H, 13C, 15N resonance assignments of rat lipocalin2" Biomol. NMR Assignments 3, 95-97 (2009).
PubMed: 19636955
Assembly members:
rat_lipocalin_2, polymer, 188 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet22b
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 647 |
| 15N chemical shifts | 175 |
| 1H chemical shifts | 1168 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | whole | 1 |
Entities:
Entity 1, whole 188 residues - Formula weight is not available
| 1 | GLY | SER | GLN | ASP | SER | THR | GLN | ASN | LEU | ILE | ||||
| 2 | PRO | ALA | PRO | PRO | LEU | ILE | SER | VAL | PRO | LEU | ||||
| 3 | GLN | PRO | GLY | PHE | TRP | THR | GLU | ARG | PHE | GLN | ||||
| 4 | GLY | ARG | TRP | PHE | VAL | VAL | GLY | LEU | ALA | GLY | ||||
| 5 | ASN | ALA | VAL | GLN | LYS | GLU | ARG | GLN | SER | ARG | ||||
| 6 | PHE | THR | MET | TYR | SER | THR | ILE | TYR | GLU | LEU | ||||
| 7 | GLN | GLU | ASP | ASN | SER | TYR | ASN | VAL | THR | SER | ||||
| 8 | ILE | LEU | VAL | ARG | GLY | GLN | GLY | CYS | ARG | TYR | ||||
| 9 | TRP | ILE | ARG | THR | PHE | VAL | PRO | SER | SER | ARG | ||||
| 10 | PRO | GLY | GLN | PHE | THR | LEU | GLY | ASN | ILE | HIS | ||||
| 11 | SER | TYR | PRO | GLN | ILE | GLN | SER | TYR | ASP | VAL | ||||
| 12 | GLN | VAL | ALA | ASP | THR | ASP | TYR | ASP | GLN | PHE | ||||
| 13 | ALA | MET | VAL | PHE | PHE | GLN | LYS | THR | SER | GLU | ||||
| 14 | ASN | LYS | GLN | TYR | PHE | LYS | VAL | THR | LEU | TYR | ||||
| 15 | GLY | ARG | THR | LYS | GLY | LEU | SER | ASP | GLU | LEU | ||||
| 16 | LYS | GLU | ARG | PHE | VAL | SER | PHE | ALA | LYS | SER | ||||
| 17 | LEU | GLY | LEU | LYS | ASP | ASN | ASN | ILE | VAL | PHE | ||||
| 18 | SER | VAL | PRO | THR | ASP | GLN | CYS | ILE | ASP | ASN | ||||
| 19 | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: rat lipocalin 2, [U-99% 13C; U-99% 15N], 0.8 mM
sample_conditions_1: ionic strength: 0.17 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA31657 |
| GB | AAB35994 AAH89053 EDL93236 |
| REF | NP_570097 |
| SP | P30152 |
| AlphaFold | P30152 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks