BMRB Entry 15678

Title:
Chemical Shift assignment of SeR13 from Staphylococcus Epidermidis, North East Structural Genomics Consortium Target SeR13
Deposition date:
2008-03-05
Original release date:
2008-03-17
Authors:
Lee, Hsiau-Wei; Wylie, Greg; Bansal, Sonal; Wang, Xu; Shastry, Ritu; Jiang, Mei; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, GVT; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Prestegard, James
Citation:

Citation: Lee, Hsiau-Wei; Wylie, Greg; Bansal, Sonal; Wang, Xu; Barb, Adam; Macnaughtan, Megan; Ertekin, Asli; Montelione, Gaetano; Prestegard, James. "Three-dimensional structure of the weakly associated protein homodimer SeR13 using RDCs and paramagnetic surface mapping."  Protein Sci. 19, 1673-1685 (2010).
PubMed: 20589905

Assembly members:

Assembly members:
SeR13, polymer, 94 residues, 10138.311 Da.

Natural source:

Natural source:   Common Name: Staphylococcus epidermidis   Taxonomy ID: 1282   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus epidermidis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Staphylococcus epidermidis   Vector: pET21

Data sets:
Data typeCount
13C chemical shifts345
15N chemical shifts85
1H chemical shifts494
residual dipolar couplings106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SeR131

Entities:

Entity 1, SeR13 94 residues - 10138.311 Da.

LEHHHHHH are at the C-terminal as part of His-Tag

1   METGLUILEILEALAILESERGLUTHRPRO
2   ASNHISASNTHRMETLYSVALSERLEUSER
3   GLUPROARGGLNASPASNSERPHETHRTHR
4   TYRTHRALAALAGLNGLUGLYGLNPROGLU
5   PHEILEASNARGLEUPHEGLUILEGLUGLY
6   VALLYSSERILEPHETYRVALLEUASPPHE
7   ILESERILEASPLYSGLUASPASNALAASN
8   TRPASNGLULEULEUPROGLNILEGLUASN
9   THRPHEALALYSSERASNLEUGLUHISHIS
10   HISHISHISHIS

Related Database Links:

PDB 2K1H
EMBL CDM13704
GB AAO04721 AAW54376 AIR82327 AJP24693 EES36166
REF NP_764679 WP_001831110 WP_002489988 WP_002493609 WP_047500344

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks