BMRB Entry 15666

Name: Mutant Collision E7 protein IM7 with an extended helix III in M Urea
Published: 2009-11-10

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15666

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mutant Collision E7 protein IM7 with an extended helix III in M Urea

Deposition date:

2008-02-20

Original release date:

2009-11-10

Authors:

Figueiredo, Angelo; Whittaker, Sara; Knowling, Stuart; Radford, Sheena; Moore, Geoffrey

Citation:

Citation: Knowling, Stuart; Figueiredo, Angelo Miguel; Whittaker, Sara B-M; Moore, Geoffrey; Radford, Sheena. "Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin E7 immunity protein Im7." J. Mol. Biol. 392, 1074-1086 (2009).
PubMed: 19651139

Assembly members:

Assembly members:
Im7_mutant, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: JM109

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Im7_mutant: MEHHHHHHELKNSISDYTEA EFVQLLKEIEKENVAATDDV LDVLLEHFVKITEHPDGTAL IYEAAARAAANPGGDGGGPE GIVKEIKEWRAANGKPGFKQ G

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	175
15N chemical shifts	88
1H chemical shifts	88

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Im7 mutant	1

Entities:

Entity 1, Im7 mutant 101 residues - Formula weight is not available

1

MET

GLU

HIS

GLU

LEU

2

LYS

ASN

SER

ILE

SER

ASP

TYR

THR

GLU

ALA

3

GLU

PHE

VAL

GLN

LEU

LYS

GLU

ILE

GLU

4

LYS

GLU

ASN

VAL

ALA

THR

ASP

VAL

5

LEU

ASP

VAL

LEU

GLU

HIS

PHE

VAL

LYS

6

ILE

THR

GLU

HIS

PRO

ASP

GLY

THR

ALA

LEU

7

ILE

TYR

GLU

ALA

ARG

ALA

8

ASN

PRO

GLY

ASP

GLY

PRO

GLU

9

GLY

ILE

VAL

LYS

GLU

ILE

LYS

GLU

TRP

ARG

10

ALA

ASN

GLY

LYS

PRO

GLY

PHE

LYS

GLN

11

GLY

Samples:

sample_1: Im7 mutant, [U-13C; U-15N], 1 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH*: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.1.1, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 800 MHz
Varian INOVA 500 MHz

BMRB	15645
PDB	2K0D
GB	EYB47026