BMRB Entry 15665

Name: CONFIRMATIONAL ANALYSIS OF THE BROAD-SPECTRUM ANTIBACTERIAL PEPTIDE, RANTUERIN-2CSA: IDENTIFICATION OF A FULL LENGTH HELIX-TURN-HELIX MOTIF
Published: 2008-06-27

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PDB ID: 2k10
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15665
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CONFIRMATIONAL ANALYSIS OF THE BROAD-SPECTRUM ANTIBACTERIAL PEPTIDE, RANTUERIN-2CSA: IDENTIFICATION OF A FULL LENGTH HELIX-TURN-HELIX MOTIF

Deposition date:

2008-02-19

Original release date:

2008-06-27

Authors:

Hewage, Chandralal; Subasinghage, Anusha; Conlon, Michael

Citation:

Citation: Subasinghage, Anusha; Conlon, J.; Hewage, Chandralal. "Conformational analysis of the broad-spectrum antibacterial peptide, ranatuerin-2CSa: Identification of a full length helix-turn-helix motif." Biochim. Biophys. Acta 1784, 924-929 (2008).
PubMed: 18387372

Assembly members:

Assembly members:
ranatuerin-2CSa, polymer, 32 residues, 3256.000 Da.

Natural source:

Natural source: Common Name: Rana cascadae Taxonomy ID: 160497 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana cascadae

Experimental source:

Experimental source: Production method: chemical synthesis Host organism: Rana cascadae

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ranatuerin-2CSa: GILSSFKGVAKGVAKDLAGK LLETLKCKITGC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	215

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ranatuerin-2CSa	1

Entities:

Entity 1, ranatuerin-2CSa 32 residues - 3256.000 Da.

1

GLY

ILE

LEU

SER

PHE

LYS

GLY

VAL

ALA

2

LYS

GLY

VAL

ALA

LYS

ASP

LEU

ALA

GLY

LYS

3

LEU

GLU

THR

LEU

LYS

CYS

LYS

ILE

THR

4

GLY

CYS

Samples:

Ranatuerin-2CSa: Peptide 1.5-2.0 mM; TFE-d3/H2O 1:1 v/v

Ranatuerin-2CSa_c: pH: 3.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	Ranatuerin-2CSa	isotropic	Ranatuerin-2CSa_c
2D 1H-1H NOESY	Ranatuerin-2CSa	isotropic	Ranatuerin-2CSa_c
2D DQF-COSY	Ranatuerin-2CSa	isotropic	Ranatuerin-2CSa_c

Software:

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - processing

SYBYL v6.8.1, Tripos - refinement

xwinnmr v3.5, Bruker Biospin - collection

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 800 MHz