BMRB Entry 15663

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15663
MolProbity Validation Chart

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Title:
Almost Complete Chemical Shift Assignments for a new calcium binding protein, EhCaM
Deposition date:
2008-02-19
Original release date:
2008-04-14
Authors:
Rout, Ashok K; Barnwal, Ravi P; Chary, Kandala V R
Citation:

Citation: Rout, Ashok; Barnwal, Ravi; Padhan, Narendra; Bhattacharya, Alok; Chary, Kandala. "Sequence specific 1H, 13C and 15N resonance assignments of a calmodulin-like calcium-binding protein from the protozoan parasite Entamoeba histolytica (EhCaM)"  Biomol. NMR Assignments 2, 77-79 (2008).
PubMed: 19636930

Assembly members:

Assembly members:
Calcium_Binding_Protein_3, polymer, 151 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calcium_Binding_Protein_3: MSEQKKVLTAEEQQEYKEAF QLFDKDNDNKLTAEELGTVM RALGANPTKQKISEIVKDYD KDNSGKFDQETFLTIMLEYG QEVDSTEDIKKAFEIFDKEK NGYISASELKHVLTTLGEKL TEQEVDDLLKEIGVEEGLIN VDDFVKLITSK

Data sets:
Data typeCount
13C chemical shifts561
15N chemical shifts135
1H chemical shifts690

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2Calcium ion2

Entities:

Entity 1, subunit 1 151 residues - Formula weight is not available

1   METSERGLUGLNLYSLYSVALLEUTHRALA
2   GLUGLUGLNGLNGLUTYRLYSGLUALAPHE
3   GLNLEUPHEASPLYSASPASNASPASNLYS
4   LEUTHRALAGLUGLULEUGLYTHRVALMET
5   ARGALALEUGLYALAASNPROTHRLYSGLN
6   LYSILESERGLUILEVALLYSASPTYRASP
7   LYSASPASNSERGLYLYSPHEASPGLNGLU
8   THRPHELEUTHRILEMETLEUGLUTYRGLY
9   GLNGLUVALASPSERTHRGLUASPILELYS
10   LYSALAPHEGLUILEPHEASPLYSGLULYS
11   ASNGLYTYRILESERALASERGLULEULYS
12   HISVALLEUTHRTHRLEUGLYGLULYSLEU
13   THRGLUGLNGLUVALASPASPLEULEULYS
14   GLUILEGLYVALGLUGLUGLYLEUILEASN
15   VALASPASPPHEVALLYSLEUILETHRSER
16   LYS

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: EhCaM, [U-99% 15N], 1.1 mM; calcium2+ 30 mM

sample_2: EhCaM, [U-95% 13C], 1.2 mM; calcium2+ 30 mM

sample_3: EhCaM, [U-99% 13C; U-99% 15N], 1.1 mM; calcium2+ 30 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HN(CA)COsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.0, Bruker Biospin - collection

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB EAL46322 EDR29145 EKE39237 EMD47360 EMH75082
REF XP_001734679 XP_008858434 XP_651708

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks