BMRB Entry 15662

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15662

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Title:
1H, 13C, and 15N resonance assignments of murine amelogenin, an essential enamel biomineralization protein.
Deposition date:
2008-02-18
Original release date:
2008-02-22
Authors:
Buchko, Garry; Bekhazi, Jacky; Cort, John; Valentine, Nancy; Shaw, Wendy
Citation:

Citation: Buchko, Garry; Bekhazi, Jacky; Cort, John; Valentine, Nancy; Snead, Malcolm; Shaw, Wendy. "1H, 13C, and 15N resonance assignments of murine amelogenin, an essential enamel biomineralization protein"  Biomol. NMR Assignments 2, 89-91 (2008).
PubMed: 19081741

Assembly members:

Assembly members:
amelogenin, polymer, 191 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
amelogenin: MRGSHHHHHHGSPLPPHPGS PGYINLSYEVLTPLKWYQSM IRQPYPSYGYEPMGGWLHHQ IIPVLSQQHPPSHTLQPHHH LPVVPAQQPVAPQQPMMPVP GHHSMTPTQHHQPNIPPSAQ QPFQQPFQPQAIPPQSHQPM QPQSPLHPMQPLAPQPPLPP LFSMQPLSPILPELPLEAWP ATDKTKREEVD

Data sets:
Data typeCount
13C chemical shifts641
15N chemical shifts142
1H chemical shifts687

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1amelogenin1

Entities:

Entity 1, amelogenin 191 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERPROLEUPROPROHISPROGLYSER
3   PROGLYTYRILEASNLEUSERTYRGLUVAL
4   LEUTHRPROLEULYSTRPTYRGLNSERMET
5   ILEARGGLNPROTYRPROSERTYRGLYTYR
6   GLUPROMETGLYGLYTRPLEUHISHISGLN
7   ILEILEPROVALLEUSERGLNGLNHISPRO
8   PROSERHISTHRLEUGLNPROHISHISHIS
9   LEUPROVALVALPROALAGLNGLNPROVAL
10   ALAPROGLNGLNPROMETMETPROVALPRO
11   GLYHISHISSERMETTHRPROTHRGLNHIS
12   HISGLNPROASNILEPROPROSERALAGLN
13   GLNPROPHEGLNGLNPROPHEGLNPROGLN
14   ALAILEPROPROGLNSERHISGLNPROMET
15   GLNPROGLNSERPROLEUHISPROMETGLN
16   PROLEUALAPROGLNPROPROLEUPROPRO
17   LEUPHESERMETGLNPROLEUSERPROILE
18   LEUPROGLULEUPROLEUGLUALATRPPRO
19   ALATHRASPLYSTHRLYSARGGLUGLUVAL
20   ASP

Samples:

sample_1: amelogenin, [U-99% 13C; U-99% 15N], 2 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

sample_2: amelogenin, 50% [U-15N]-Leu, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_3: amelogenin, 50% [U-15N]-Val, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_4: amelogenin, 50% [U-15N]-His, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_5: amelogenin, 50% [U-15N]-Arg, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_6: amelogenin, 50% [U-15N]-Lys, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_7: amelogenin, 50% [U-15N]-Met, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_8: amelogenin, 50% [U-15N]-Phe, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_9: amelogenin, 50% [U-15N]-Tyr, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

Sample_10: amelogenin, 50% [U-15N]-Glu/Gln, 0.2-1 ± 0.2 mM; acetic acid, [U-2H], 2 ± 0.1 %

sample_conditions_1: ionic strength: 0 M; pH: 3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCSample_3isotropicsample_conditions_1
2D 1H-15N HSQCSample_4isotropicsample_conditions_1
2D 1H-15N HSQCSample_5isotropicsample_conditions_1
2D 1H-15N HSQCSample_6isotropicsample_conditions_1
2D 1H-15N HSQCSample_7isotropicsample_conditions_1
2D 1H-15N HSQCSample_8isotropicsample_conditions_1
2D 1H-15N HSQCSample_9isotropicsample_conditions_1
2D 1H-15N HSQCSample_10isotropicsample_conditions_1

Software:

FELIX v2007, Accelrys Software Inc. - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 750 MHz

Related Database Links:

DBJ BAA06546 BAC26415
GB AAA20491 AAA37218 AAB02691 AAH59090 ABY48714
REF NP_001075447 NP_001258003 NP_001258006 NP_033796 XP_003503248

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks