BMRB Entry 15653

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15653

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Title:
Backbone 1H, 13C, and 15N NMR Assignments of the Tail Domain of Vinculin
Deposition date:
2008-02-04
Original release date:
2008-05-28
Authors:
Palmer, Sean; Campbell, Sharon
Citation:

Citation: Palmer, Sean; Campbell, Sharon. "Backbone 1H, 13C, and 15N NMR assignments of the tail domain of vinculin"  Biomol. NMR Assignments 2, 69-71 (2008).
PubMed: 19636928

Assembly members:

Assembly members:
Vinculin_Tail, polymer, 192 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Vinculin_Tail: GSHMEEKDEEFPEQKAGEAI NQPMMMAARQLHDEARKWSS KGNDIIAAAKRMALLMAEMS RLVRGGSGNKRALIQCAKDI AKASDEVTRLAKEVAKQCTD KRIRTNLLQVCERIPTISTQ LKILSTVKATMLGRTNISDE ESEQATEMLVHNAQNLMQSV KETVREAEAASIKIRTDAGF TLRWVRKTPWYQ

Data sets:
Data typeCount
13C chemical shifts486
15N chemical shifts155
1H chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vinculin Tail1

Entities:

Entity 1, Vinculin Tail 192 residues - Formula weight is not available

Residues 1-4 are non-native. Residues 5-192 represent residues 879-1066 of chicken vinculin, commonly refered to as vinculin tail (Vt). Chicken Vt shares 98.9% identity with human Vt.

1   GLYSERHISMETGLUGLULYSASPGLUGLU
2   PHEPROGLUGLNLYSALAGLYGLUALAILE
3   ASNGLNPROMETMETMETALAALAARGGLN
4   LEUHISASPGLUALAARGLYSTRPSERSER
5   LYSGLYASNASPILEILEALAALAALALYS
6   ARGMETALALEULEUMETALAGLUMETSER
7   ARGLEUVALARGGLYGLYSERGLYASNLYS
8   ARGALALEUILEGLNCYSALALYSASPILE
9   ALALYSALASERASPGLUVALTHRARGLEU
10   ALALYSGLUVALALALYSGLNCYSTHRASP
11   LYSARGILEARGTHRASNLEULEUGLNVAL
12   CYSGLUARGILEPROTHRILESERTHRGLN
13   LEULYSILELEUSERTHRVALLYSALATHR
14   METLEUGLYARGTHRASNILESERASPGLU
15   GLUSERGLUGLNALATHRGLUMETLEUVAL
16   HISASNALAGLNASNLEUMETGLNSERVAL
17   LYSGLUTHRVALARGGLUALAGLUALAALA
18   SERILELYSILEARGTHRASPALAGLYPHE
19   THRLEUARGTRPVALARGLYSTHRPROTRP
20   TYRGLN

Samples:

sample_1: Vinculin Tail, [U-13C; U-15N; U-2H], 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_2: Vinculin Tail, [U-13C; U-15N], 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_3: Vinculin Tail, [U-15N]-Leu, 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_4: Vinculin Tail, [U-15N]-Met, 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_5: Vinculin Tail, [U-15N; NA-Lys], 0.3 mM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 2 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1

Software:

NMRView v5.2.2.01, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe/NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 700 MHz
  • Varian INOVA 800 MHz

Related Database Links:

GB NP_990772 AAA49136 AAA61283 AAB21657 AAB96843 AAH08520
PDB
DBJ BAC28973 BAC37033 BAG11028 BAG54414 BAG59141
PRF 1805212A
REF NP_001100718 NP_003364 NP_033528 NP_990772 NP_999099
SP P85972 Q64727
TPG DAA14174
AlphaFold P85972 Q64727

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks