BMRB Entry 15646

Name: NMR analysis of the closed conformation of syntaxin-1
Published: 2008-02-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15646

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR analysis of the closed conformation of syntaxin-1

Deposition date:

2008-02-02

Original release date:

2008-02-05

Authors:

Chen, Xiaocheng; Lu, Jun; Dulubova, Irina

Citation:

Citation: Chen, Xiaocheng; Lu, Jun; Dulubova, Irina; Rizo, Josep. "NMR analysis of the closed conformation of syntaxin-1" J. Biomol. NMR 41, 43-54 (2008).
PubMed: 18458823

Assembly members:

Assembly members:
sx23, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX-KT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sx23: GSRDRFMDEFFEQVEEIRGF IDKIAENVEEVKRKHSAILA SPNPDEKTKEELEELMSDIK KTANKVRSKLKSIEQSIEQE EGLNRSSADLRIRKTQHSTL SRKFVEVMSEYNATQSDYRE RCKGRIQRQLEITGRTTTSE ELEDMLESGNPAIFASGIIM DSSISKQALSEIETRHSEII KLENRLRELHDMFMDMAMLV ESQGEMI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	605
15N chemical shifts	151
1H chemical shifts	811

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sx23	1

Entities:

Entity 1, sx23 207 residues - Formula weight is not available

1

GLY

SER

ARG

ASP

ARG

PHE

MET

ASP

GLU

PHE

2

PHE

GLU

GLN

VAL

GLU

ILE

ARG

GLY

PHE

3

ILE

ASP

LYS

ILE

ALA

GLU

ASN

VAL

GLU

4

VAL

LYS

ARG

LYS

HIS

SER

ALA

ILE

LEU

ALA

5

SER

PRO

ASN

PRO

ASP

GLU

LYS

THR

LYS

GLU

6

GLU

LEU

GLU

LEU

MET

SER

ASP

ILE

LYS

7

LYS

THR

ALA

ASN

LYS

VAL

ARG

SER

LYS

LEU

8

LYS

SER

ILE

GLU

GLN

SER

ILE

GLU

GLN

GLU

9

GLU

GLY

LEU

ASN

ARG

SER

ALA

ASP

LEU

10

ARG

ILE

ARG

LYS

THR

GLN

HIS

SER

THR

LEU

11

SER

ARG

LYS

PHE

VAL

GLU

VAL

MET

SER

GLU

12

TYR

ASN

ALA

THR

GLN

SER

ASP

TYR

ARG

GLU

13

ARG

CYS

LYS

GLY

ARG

ILE

GLN

ARG

GLN

LEU

14

GLU

ILE

THR

GLY

ARG

THR

SER

GLU

15

GLU

LEU

GLU

ASP

MET

LEU

GLU

SER

GLY

ASN

16

PRO

ALA

ILE

PHE

ALA

SER

GLY

ILE

MET

17

ASP

SER

ILE

SER

LYS

GLN

ALA

LEU

SER

18

GLU

ILE

GLU

THR

ARG

HIS

SER

GLU

ILE

19

LYS

LEU

GLU

ASN

ARG

LEU

ARG

GLU

LEU

HIS

20

ASP

MET

PHE

MET

ASP

MET

ALA

MET

LEU

VAL

21

GLU

SER

GLN

GLY

GLU

MET

ILE

Samples:

sample_1: sx23, [U-99% 13C; U-99% 15N], 700 uM; sodium phosphate 20 mM

sample_2: sx23, [U-99% 15N], 700 uM; sodium phosphate 20 mM

sample_3: sx23, [U-13C; U-15N; U-2H], 700 uM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 301 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HN(CO)CA	sample_3	isotropic	sample_conditions_1

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	4198
PDB	1BR0 1EZ3 3C98 4JEH 4JEU
DBJ	BAA01231 BAA02089 BAA07151 BAA28865 BAC78519
EMBL	CAG33299 CAH93304
GB	AAA20940 AAA42195 AAA53519 AAB22525 AAB65500
PIR	G01485
PRF	2116295A
REF	NP_001028037 NP_001126944 NP_001159375 NP_004594 NP_058081
SP	O35526 P32850 P32851 Q16623 Q5R4L2
AlphaFold	O35526 P32850 P32851 Q16623 Q5R4L2