BMRB Entry 15621

Title:
Cam-HrTH-I
Deposition date:
2008-01-08
Original release date:
2008-06-27
Authors:
Munte, Claudia; Gaede, Gerd; Domogalla, Barbara; Kremer, Werner; Kellner, Roland; Kalbitzer, Hans Robert
Citation:

Citation: Munte, Claudia; Gaede, Gerd; Domogalla, Barbara; Kremer, Werner; Kellner, Roland; Kalbitzer, H.. "C-mannosylation in the hypertrehalosaemic hormone from the stick insect Carausius morosus"  FEBS. J. 275, 1163-1173 (2008).
PubMed: 18266868

Assembly members:

Assembly members:
Cam-HrTH-I, polymer, 10 residues, Formula weight is not available
MAN, non-polymer, 180.156 Da.

Natural source:

Natural source:   Common Name: Indian stick insect   Taxonomy ID: 7022   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Carausius morosus

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Carausius morosus

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cam-HrTH-I: ELTFTPNWGT

Data sets:
Data typeCount
13C chemical shifts37
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2apha-D-mannopyranose2

Entities:

Entity 1, subunit 1 10 residues - Formula weight is not available

1   GLULEUTHRPHETHRPROASNTRPGLYTHR

Entity 2, apha-D-mannopyranose - C6 H12 O6 - 180.156 Da.

1   MAN

Samples:

sample_1: Cam-HrTH-I 60 uM; H2O 90%; D2O, [U-99% 2H], 10%; DSS 100 uM

sample_2: Cam-HrTH-I 60 uM; D2O, [U-99% 2H], 100%; DSS 100%

sample_conditions_1: pH: 5.4; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H ROESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

AUREMOL, Bruker Biospin - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz