BMRB Entry 15621
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15621
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Citation: Munte, Claudia; Gaede, Gerd; Domogalla, Barbara; Kremer, Werner; Kellner, Roland; Kalbitzer, H.. "C-mannosylation in the hypertrehalosaemic hormone
from the stick insect Carausius morosus" FEBS. J. 275, 1163-1173 (2008).
PubMed: 18266868
Assembly members:
Cam-HrTH-I, polymer, 10 residues, Formula weight is not available
MAN, non-polymer, 180.156 Da.
Natural source: Common Name: Indian stick insect Taxonomy ID: 7022 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Carausius morosus
Experimental source: Production method: purified from the natural source Host organism: Carausius morosus
Entity Sequences (FASTA):
Cam-HrTH-I: ELTFTPNWGT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 37 |
| 1H chemical shifts | 53 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
| 2 | apha-D-mannopyranose | 2 |
Entities:
Entity 1, subunit 1 10 residues - Formula weight is not available
| 1 | GLU | LEU | THR | PHE | THR | PRO | ASN | TRP | GLY | THR |
Entity 2, apha-D-mannopyranose - C6 H12 O6 - 180.156 Da.
| 1 | MAN |
Samples:
sample_1: Cam-HrTH-I 60 uM; H2O 90%; D2O, [U-99% 2H], 10%; DSS 100 uM
sample_2: Cam-HrTH-I 60 uM; D2O, [U-99% 2H], 100%; DSS 100%
sample_conditions_1: pH: 5.4; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
AUREMOL, Bruker Biospin - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz