BMRB Entry 15614

Name: Solution structure of the complex between E.coli NusA-AR2 and RNAP-aCTD
Published: 2009-11-03

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PDB ID: 2jzb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15614
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the complex between E.coli NusA-AR2 and RNAP-aCTD

Deposition date:

2008-01-02

Original release date:

2009-11-03

Authors:

Prasch, Stefan; Schweimer, Kristian; Roesch, Paul

Citation:

Citation: Prasch, Stefan; Schweimer, Kristian; Roesch, Paul. "structural basis of transcription elongation control: the NusA-aCTD complex" .

Assembly members:

Assembly members:
actd, polymer, 99 residues, 9014.511 Da.
ar2, polymer, 74 residues, 7470.336 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET19b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
actd: GPDLRDVRQPEVKEEKPEFD PILLRPVDDLELTVRSANCL KAEAIHYIGDLVQRTEVELL KTPNLGKKSLTEIKDVLASR GLSLGMRLENWPPASIADE
ar2: GPSLGDNKPADDLLNLEGVD RDLAFKLAARGVCTLEDLAE QGIDDLADIEGLTDEKAGAL IMAARNICWFGDEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	540
15N chemical shifts	170
1H chemical shifts	1176

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 99 residues - 9014.511 Da.

1

GLY

PRO

ASP

LEU

ARG

ASP

VAL

ARG

GLN

PRO

2

GLU

VAL

LYS

GLU

LYS

PRO

GLU

PHE

ASP

3

PRO

ILE

LEU

ARG

PRO

VAL

ASP

LEU

4

GLU

LEU

THR

VAL

ARG

SER

ALA

ASN

CYS

LEU

5

LYS

ALA

GLU

ALA

ILE

HIS

TYR

ILE

GLY

ASP

6

LEU

VAL

GLN

ARG

THR

GLU

VAL

GLU

LEU

7

LYS

THR

PRO

ASN

LEU

GLY

LYS

SER

LEU

8

THR

GLU

ILE

LYS

ASP

VAL

LEU

ALA

SER

ARG

9

GLY

LEU

SER

LEU

GLY

MET

ARG

LEU

GLU

ASN

10

TRP

PRO

ALA

SER

ILE

ALA

ASP

GLU

Entity 2, entity_2 74 residues - 7470.336 Da.

1

GLY

PRO

SER

LEU

GLY

ASP

ASN

LYS

PRO

ALA

2

ASP

LEU

ASN

LEU

GLU

GLY

VAL

ASP

3

ARG

ASP

LEU

ALA

PHE

LYS

LEU

ALA

ARG

4

GLY

VAL

CYS

THR

LEU

GLU

ASP

LEU

ALA

GLU

5

GLN

GLY

ILE

ASP

LEU

ALA

ASP

ILE

GLU

6

GLY

LEU

THR

ASP

GLU

LYS

ALA

GLY

ALA

LEU

7

ILE

MET

ALA

ARG

ASN

ILE

CYS

TRP

PHE

8

GLY

ASP

GLU

ALA

Samples:

sample_1: actd, [U-98% 13C; U-98% 15N], 0.7 mM; ar2 2.1 mM; potassium phosphate 10 mM; sodium chloride 50 mM; beta-mercaptoethanol 1 mM

sample_2: ar2, [U-98% 13C; U-98% 15N], 0.7 mM; actd 2.1 mM; potassium phosphate 10 mM; sodium chloride 50 mM; beta-mercaptoethanol 1 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 800 MHz

PDB	1COO 1LB2 1XS9 2JZB 3IYD 3LU0 3N4M 3N97 4JK1 4JK2 4JKR 4KMU 4KN4 4KN7 4MEX 4MEY 4S20 4YFK 4YFN 4YFX 4YG2 4ZH2 4ZH3 4ZH4 5BYH 1WCN 2JZB
DBJ	BAA11840 BAB37583 BAE75528 BAE77996 BAG79094 BAB37473 BAE77215 BAG78979 BAI27449 BAI32628
EMBL	CAA25337 CAA26395 CAA37838 CAA37839 CAD09171 CAA25200 CAP77631 CAQ33504 CAQ90641 CAR00133
GB	AAA24577 AAA27214 AAA58092 AAC76320 AAG58416 AAA57972 AAC76203 AAG58305 AAN44677 AAN82367
PIR	AB1009
REF	NP_312187 NP_417754 NP_458485 NP_462319 NP_709083 NP_289745 NP_312077 NP_417638 NP_708970 NP_755793
SP	A0KF45 A1AGI6 A1JS01 A4SSY1 A4TH15 P0AFF6 P0AFF7 P0AFF8 P0AFF9
BMRB	5800
AlphaFold	A0KF45 A1AGI6 A1JS01 A4SSY1 A4TH15 P0AFF6 P0AFF7 P0AFF8 P0AFF9