BMRB Entry 15601

Title:
Chemical shift assignments for Atra-PBP1
Deposition date:
2007-12-18
Original release date:
2008-06-05
Authors:
Ames, James
Citation:

Citation: Xu, Xianzhong; Li, Yun; Rayo, Josep; Ishida, Yuko; Leal, Walter; Ames, James. "1H, 15N, and 13C Chemical shift assignments of the navel orange worm pheromone-binding protein-1 (Atra-PBP1)"  Biomol. NMR Assignments 2, 105-106 (2008).
PubMed: 19636880

Assembly members:

Assembly members:
Atra-PBP1, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: polyphemus moth   Taxonomy ID: 7120   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Antheraea Polyphemus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts409
15N chemical shifts135
1H chemical shifts521

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 142 residues - Formula weight is not available

1   SERPROGLUILEMETLYSASPLEUSERILE
2   ASNPHEGLYLYSALALEUASPTHRCYSLYS
3   LYSGLULEUASPLEUPROASPSERILEASN
4   GLUASPPHETYRLYSPHETRPLYSGLUASP
5   TYRGLUILETHRASNARGLEUTHRGLYCYS
6   ALAILELYSCYSLEUSERGLULYSLEUGLU
7   METVALASPALAASPGLYLYSLEUHISHIS
8   GLYASNALAARGGLUPHEALAMETLYSHIS
9   GLYALAASPASPALAMETALALYSGLNLEU
10   VALASPLEUILEHISGLYCYSGLULYSSER
11   ILEPROPROASNASPASPARGCYSMETGLU
12   VALLEUSERILEALAMETCYSPHELYSLYS
13   GLUILEHISASNLEULYSTRPALAPROASN
14   METGLUVALVALVALGLYGLUVALLEUALA
15   GLUVAL

Samples:

sample_1: Atra-PBP1, [U-100% 13C; U-100% 15N], 1 mM; D2O, [U-100% 2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 0.1 M; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB ACX47890
REF NP_001299593

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks