BMRB Entry 15595

Name: A novel solution NMR structure of protein yst0336 from Saccharomyces/Northest Structural Genomics Consortium Target YT51/Ontario Center for Structural Proteomics
Published: 2008-01-15

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15595

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A novel solution NMR structure of protein yst0336 from Saccharomyces/Northest Structural Genomics Consortium Target YT51/Ontario Center for Structural Proteomics

Deposition date:

2007-12-14

Original release date:

2008-01-15

Authors:

Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Gutmanas, Aleksandras; Semest, Anthony; Arrowsmith, Cheryl

Citation:

Citation: Wu, Adelinda; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Gutmanas, Aleksandras; Semest, Anthony; Arrowsmith, Cheryl. "A Novel solution NMR structure of protein yst0336 from Saccharomyces cerevisiae/North Structural Genomics Consortium Target YT51/Ontario Center for Structural Proteomics Target yst0336" .

Assembly members:

Assembly members:
yst0336, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yst0336: MSTFNAETADNLEDIEKQFA VVAVEQAETYWKLLTSVPGS KLRLTKFDDEIYENFMERFP EYKDVERVKKFTEEELKTKE AKERWRKFFTIFEKKIEDYN FGTLLRTDASAEYGQFTTCF VVRLQFYAFEIARNKHGLND WIVGQK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	627
15N chemical shifts	155
1H chemical shifts	1038

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yst0336	1

Entities:

Entity 1, yst0336 146 residues - Formula weight is not available

1

MET

SER

THR

PHE

ASN

ALA

GLU

THR

ALA

ASP

2

ASN

LEU

GLU

ASP

ILE

GLU

LYS

GLN

PHE

ALA

3

VAL

ALA

VAL

GLU

GLN

ALA

GLU

THR

TYR

4

TRP

LYS

LEU

THR

SER

VAL

PRO

GLY

SER

5

LYS

LEU

ARG

LEU

THR

LYS

PHE

ASP

GLU

6

ILE

TYR

GLU

ASN

PHE

MET

GLU

ARG

PHE

PRO

7

GLU

TYR

LYS

ASP

VAL

GLU

ARG

VAL

LYS

8

PHE

THR

GLU

LEU

LYS

THR

LYS

GLU

9

ALA

LYS

GLU

ARG

TRP

ARG

LYS

PHE

THR

10

ILE

PHE

GLU

LYS

ILE

GLU

ASP

TYR

ASN

11

PHE

GLY

THR

LEU

ARG

THR

ASP

ALA

SER

12

ALA

GLU

TYR

GLY

GLN

PHE

THR

CYS

PHE

13

VAL

ARG

LEU

GLN

PHE

TYR

ALA

PHE

GLU

14

ILE

ALA

ARG

ASN

LYS

HIS

GLY

LEU

ASN

ASP

15

TRP

ILE

VAL

GLY

GLN

LYS

Samples:

sample_1: unknown function protein yst0336 from Saccharomyces cerevisiae, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.01%; benzamidine 10 mM

sample_2: unknown function protein yst0336 from Saccharomyces cerevisiae, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.01%; benzamidine 10 mM

sample_3: unknown function protein yst0336 from Saccharomyces cerevisiae, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.01%; benzamidine 10 mM

sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.95, Goddard - data analysis, peak picking

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	2JYN
DBJ	GAA26753
EMBL	CAA97940 CAY86735
GB	AAT92581 AHY77958 AJP41926 AJU23370 AJU24058
REF	NP_015099
SP	Q08971
TPG	DAA11211
AlphaFold	Q08971