BMRB Entry 15582
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15582
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Citation: Brass, Volker; Berke, Jan Martin; Montserret, Roland; Blum, Hubert; Penin, Francois; Moradpour, Darius. "Structural determinants for membrane association and dynamic organization of the hepatitis C virus NS3-4A complex" Proc. Natl. Acad. Sci. U. S. A. 105, 14545-14550 (2008).
PubMed: 18799730
Assembly members:
NS3(10-24), polymer, 15 residues, 1562.886 Da.
Natural source: Common Name: Hepatitis C Virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepatitis C virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
NS3(10-24): TRGLLGCIITSLTGR
- assigned_chemical_shifts
- conformer_family_coord_set
| Data type | Count |
| 13C chemical shifts | 44 |
| 1H chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 15 residues - 1562.886 Da.
| 1 | THR | ARG | GLY | LEU | LEU | GLY | CYS | ILE | ILE | THR | ||||
| 2 | SER | LEU | THR | GLY | ARG |
Samples:
sample_1: NS3(10-24) 1.5 ± 0.1 mM; Sodium Dodecyl Sulfate, [U-100% 2H], 50 mM
sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMR spectrometers:
- Varian UnityPlus 500 MHz