BMRB Entry 15580
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15580
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Citation: Brass, Volker; Berke, Jan Martin; Montserret, Roland; Blum, Hubert; Penin, Francois; Moradpour, Darius. "Structural determinants for membrane association and dynamic organization of the hepatitis C virus NS3-4A complex" Proc. Natl. Acad. Sci. U. S. A. 105, 14545-14550 (2008).
PubMed: 18799730
Assembly members:
NS4A(1-21), polymer, 22 residues, 2139.488 Da.
Natural source: Common Name: Hepatitis C Virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepatitis C virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
NS4A(1-21): STWVLVGGVLAALAAYCLST
GS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 58 |
| 1H chemical shifts | 140 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 22 residues - 2139.488 Da.
| 1 | SER | THR | TRP | VAL | LEU | VAL | GLY | GLY | VAL | LEU | ||||
| 2 | ALA | ALA | LEU | ALA | ALA | TYR | CYS | LEU | SER | THR | ||||
| 3 | GLY | SER |
Samples:
sample_1: NS4A(1-21) 0.9 ± 0.1 mM; Trifluoro ethanol D2OH, [U-100% 2H], 50 % v/v; H2O 90 % v/v
sample_conditions_1: ionic strength: 0 M; pH: .; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection, processing
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMR spectrometers:
- Varian UnityPlus 500 MHz