BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15568

Title: Solution NMR Structure of uncharacterized Lipoprotein B from Nitrosomona europaea. Northeast Structural Genomics Target NeR45A.

Deposition date: 2007-11-27 Original release date: original

Authors: Rossi, Paolo; ., Wang; ., .; ., .; ., .; 7, .; Thomas, 8; Rost, Burkhard; ., Montelione

Citation: Rossi, Paolo; ., Xiao; B., .; ., T.. "Solution NMR Structure of Uncharacterized Lipoprotein B from Nitrosomona europaea. Northeast Structural Genomics Target NeR45A."  .

Assembly members:
NeR45A, polymer, 155 residues, 17617.432 Da.

Natural source:   Common Name: 15568   Taxonomy ID: 915   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology' 'Escherichia coli' . . . Escherichia coli 'BL21(DE3)+ Magic

Entity Sequences (FASTA):
NeR45A: MGFKLRGQVSELPFERVYIT APAGLTIGSDLERVISTHTR AKVVNKAEKSEAIIQIVHAI REKRILSLSESGRVREFELV YRVAARLLDAHNAELASLQE IRLTRILPFLDAQELAKAAE EEMLYKDMQKDAVQQILRQV SAFTSAGLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts650
1531H chemical shifts

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NeR45A1

Entities:

Entity 1, NeR45A 155 residues - 17617.432 Da.

UniProtKB/TrEMBL ID: Q82VF2_NITEU with trucncated transmembrane region C-term HisTag (LEHHHHHH)

1   MET.155685.GLY.1556811.
2   PRO.1556817.ILE.1556823.
3   LEU.1556829.LEU.1556835.
4   THR.1556841.VAL.1556847.
5   LYS.1556853.GLN.1556859.
6   ARG.1556865.SER.1556871.
7   ARG.1556877.LEU.1556883.
8   ALA.1556889.HIS.1556895.
9   SER.15568101.LEU.15568107.
10   PHE.15568113.LEU.15568119.
11   GLU.15568125.ASP.15568131.
12   VAL.15568137.GLN.15568143.
13   SER.15568149.HIS.15568155

Samples:

sample_1: entity 1 mM; natural abundance; . mM; .; . 6; 0.02 15568 [U-100% 2H]; . 8; ., %,

sample_2: entity 0.86 mM; natural abundance; . mM; .; . 6; 0.02 15568 [U-100% 2H]; .

sample_3: entity 1.3 mM; natural abundance; . mM; .; . 6; 0.02 15568 [U-100% 2H]; . 8; ., %,

sample_conditions_1: ionic strength: 0.1 M; 6.5: . 15568; .: atm temperature; K: 15568

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
yessotropicnot availablenot available
3not availablenot availablenot available
not availablenot available1not available
not availablenot available$sample_conditions_1not available
not availablesample_conditions_1not availablespectrometer_1
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$spectrometer_1not available
not availablespectrometer_1not availablenot available
not availablenot availablenot availablenot available
3not availablenot availablenot available
$sample_1not availablenot availablenot available
isotropicnot availablenot availablenot available
not availablenot availablenot availablenot available

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

AutoAssign v2.4, Zimmerman, Moseley, Kulikowski and Montelione - backbone chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

PSVS v1.3, Bhattacharya and Montelione - validation

SPARKY v3.112, Goddard - data analysis

TOPSPIN v1.3, Bruker Biospin - collection

Molmol v2.2, Koradi, Billeter and Wuthrich - visualization

RPF v2.1.1, Huang, Powers and Montelione - validation

Procheck, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Th - validation

MolProbity, Richardson - validation

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
CAD85049 .
. lipoprotein B transmembrane [Nitrosomonas europaea]

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts