BMRB Entry 15559

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15559
MolProbity Validation Chart

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Title:
NS4A(40-69)
Deposition date:
2007-11-19
Original release date:
2009-05-14
Authors:
Montserret, Roland; Penin, Francois
Citation:

Citation: Gouttenoire, Jerome; Castet, Valerie; Montserret, Roland; Arora, Naveen; Raussens, Vincent; Ruysschaert, Jean-Marie; Diesis, Eric; Blum, Hubert; Penin, Francois; Moradpour, Darius. "Identification of a novel determinant for membrane association in hepatitis C virus nonstructural protein 4B"  J. Virol. 83, 6257-6268 (2009).
PubMed: 19357161

Assembly members:

Assembly members:
NS4B(40-69), polymer, 30 residues, 3601.153 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 11103   Superkingdom: not available   Kingdom: Viruses   Genus/species: not available Hepacivirus

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: none   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NS4B(40-69): QTNWQKLEVFWAKHMWNFIS GIQYLAGLST

Data sets:
Data typeCount
13C chemical shifts106
1H chemical shifts226

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 30 residues - 3601.153 Da.

1   GLNTHRASNTRPGLNLYSLEUGLUVALPHE
2   TRPALALYSHISMETTRPASNPHEILESER
3   GLYILEGLNTYRLEUALAGLYLEUSERTHR

Samples:

sample_1: NS4B(40-69) 1 mM; Trifluoro Ethanol D2OH 50 % v/v; H2O 50 % v/v

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

SPARKY, Goddard - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
GB AAA45534 AAB00217 AAB00219 AAB00224 AAB66324
REF NP_671491 NP_751926
SP P27958
AlphaFold P27958