BMRB Entry 15558

Name: 1H, 13C and 15N resonance assignments of C2 domain of milk fat globule-EGF-factor 8-L
Published: 2014-03-04

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15558

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments of C2 domain of milk fat globule-EGF-factor 8-L

Deposition date:

2007-11-19

Original release date:

2014-03-04

Authors:

Vivekanandan, Subramanian; Reddy, Nanga Raviprakash; Yoon, Ho Sup

Citation:

Citation: Reddy Nanga, Ravi Prakash; Vivekanandan, Subramanian; Yoon, Ho Sup. "Expression, purification and characterization of C2 domain of milk fat globule-EGF-factor 8-L." Protein Expr. Purif. 52, 329-333 (2007).
PubMed: 17049264

Assembly members:

Assembly members:
C2_domain, polymer, 170 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2_domain: MKSGHGCSEPLGLKNNTIPD SQMSASSSYKTWNLRAFGWY PHLGRLDNQGKINAWTAQSN SAKEWLQVDLGTQRQVTGII TQGARDFGHIQYVASYKVAH SDDGVQWTVYEEQGSSKVFQ GNLDNNSHKKNIFEKPFMAR YVRVLPVSWHNRITLRLELL GCLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	595
15N chemical shifts	173
1H chemical shifts	1024

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2 domain	1

Entities:

Entity 1, C2 domain 170 residues - Formula weight is not available

1	MET	LYS	SER	GLY	HIS	GLY	CYS	SER	GLU	PRO
2	LEU	GLY	LEU	LYS	ASN	ASN	THR	ILE	PRO	ASP
3	SER	GLN	MET	SER	ALA	SER	SER	SER	TYR	LYS
4	THR	TRP	ASN	LEU	ARG	ALA	PHE	GLY	TRP	TYR
5	PRO	HIS	LEU	GLY	ARG	LEU	ASP	ASN	GLN	GLY
6	LYS	ILE	ASN	ALA	TRP	THR	ALA	GLN	SER	ASN
7	SER	ALA	LYS	GLU	TRP	LEU	GLN	VAL	ASP	LEU
8	GLY	THR	GLN	ARG	GLN	VAL	THR	GLY	ILE	ILE
9	THR	GLN	GLY	ALA	ARG	ASP	PHE	GLY	HIS	ILE
10	GLN	TYR	VAL	ALA	SER	TYR	LYS	VAL	ALA	HIS
11	SER	ASP	ASP	GLY	VAL	GLN	TRP	THR	VAL	TYR
12	GLU	GLU	GLN	GLY	SER	SER	LYS	VAL	PHE	GLN
13	GLY	ASN	LEU	ASP	ASN	ASN	SER	HIS	LYS	LYS
14	ASN	ILE	PHE	GLU	LYS	PRO	PHE	MET	ALA	ARG
15	TYR	VAL	ARG	VAL	LEU	PRO	VAL	SER	TRP	HIS
16	ASN	ARG	ILE	THR	LEU	ARG	LEU	GLU	LEU	LEU
17	GLY	CYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: C2 domain, [U-99% 13C; U-99% 15N], 0.8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.1, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

BMRB	17477
PDB	2L9L

1	MET	LYS	SER	GLY	HIS	GLY	CYS	SER	GLU	PRO
2	LEU	GLY	LEU	LYS	ASN	ASN	THR	ILE	PRO	ASP
3	SER	GLN	MET	SER	ALA	SER	SER	SER	TYR	LYS
4	THR	TRP	ASN	LEU	ARG	ALA	PHE	GLY	TRP	TYR
5	PRO	HIS	LEU	GLY	ARG	LEU	ASP	ASN	GLN	GLY
6	LYS	ILE	ASN	ALA	TRP	THR	ALA	GLN	SER	ASN
7	SER	ALA	LYS	GLU	TRP	LEU	GLN	VAL	ASP	LEU
8	GLY	THR	GLN	ARG	GLN	VAL	THR	GLY	ILE	ILE
9	THR	GLN	GLY	ALA	ARG	ASP	PHE	GLY	HIS	ILE
10	GLN	TYR	VAL	ALA	SER	TYR	LYS	VAL	ALA	HIS
11	SER	ASP	ASP	GLY	VAL	GLN	TRP	THR	VAL	TYR
12	GLU	GLU	GLN	GLY	SER	SER	LYS	VAL	PHE	GLN
13	GLY	ASN	LEU	ASP	ASN	ASN	SER	HIS	LYS	LYS
14	ASN	ILE	PHE	GLU	LYS	PRO	PHE	MET	ALA	ARG
15	TYR	VAL	ARG	VAL	LEU	PRO	VAL	SER	TRP	HIS
16	ASN	ARG	ILE	THR	LEU	ARG	LEU	GLU	LEU	LEU
17	GLY	CYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	LYS	SER	GLY	HIS	GLY	CYS	SER	GLU	PRO
2	LEU	GLY	LEU	LYS	ASN	ASN	THR	ILE	PRO	ASP
3	SER	GLN	MET	SER	ALA	SER	SER	SER	TYR	LYS
4	THR	TRP	ASN	LEU	ARG	ALA	PHE	GLY	TRP	TYR
5	PRO	HIS	LEU	GLY	ARG	LEU	ASP	ASN	GLN	GLY
6	LYS	ILE	ASN	ALA	TRP	THR	ALA	GLN	SER	ASN
7	SER	ALA	LYS	GLU	TRP	LEU	GLN	VAL	ASP	LEU
8	GLY	THR	GLN	ARG	GLN	VAL	THR	GLY	ILE	ILE
9	THR	GLN	GLY	ALA	ARG	ASP	PHE	GLY	HIS	ILE
10	GLN	TYR	VAL	ALA	SER	TYR	LYS	VAL	ALA	HIS
11	SER	ASP	ASP	GLY	VAL	GLN	TRP	THR	VAL	TYR
12	GLU	GLU	GLN	GLY	SER	SER	LYS	VAL	PHE	GLN
13	GLY	ASN	LEU	ASP	ASN	ASN	SER	HIS	LYS	LYS
14	ASN	ILE	PHE	GLU	LYS	PRO	PHE	MET	ALA	ARG
15	TYR	VAL	ARG	VAL	LEU	PRO	VAL	SER	TRP	HIS
16	ASN	ARG	ILE	THR	LEU	ARG	LEU	GLU	LEU	LEU
17	GLY	CYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS