BMRB Entry 15557

Name: 1H, 13C, and 15N Chemical Shift Assignment for actin depolymerizing and dynamics regulatory protein from Leishmania donovani
Published: 2009-11-20

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15557

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignment for actin depolymerizing and dynamics regulatory protein from Leishmania donovani

Deposition date:

2007-11-17

Original release date:

2009-11-20

Authors:

Pathak, Prem Prakash; Pulavarti, S.V.S.R. Krishna; Jain, Anupam; Sahasrabuddhe, Amogh Anant; Gupta, Chhitar Mal; Arora, Ashish

Citation:

Citation: Pathak, Prem Prakash; Pulavarti, S V S R Krishna; Jain, Anupam; Sahasrabuddhe, Amogh Anant; Gupta, Chittar Mal; Arora, Ashish. "NMR assignment of actin depolymerizing and dynamics regulatory protein from Leishmania donovani." Biomol. NMR Assignments 3, 265-267 (2009).
PubMed: 19844807

Assembly members:

Assembly members:
Actin_Depolymerzing_Factor, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Leishmania donovani Taxonomy ID: 5661 Superkingdom: Eukaryota Kingdom: not available Genus/species: Leishmania donovani

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21D

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Actin_Depolymerzing_Factor: MAISGVTLEESVRGAIDDLR MKKSRYVMMCIGADGKKIEV TEVGERSVNYTDLKEKFSTE KPCYVAFDFEYNDAGSKREK LILIQWIPDTARPREKMMYS ASRDALSSVSEGYLPIQAND ESGLDAEEIIRKVRLHRSVA AALEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	571
15N chemical shifts	141
1H chemical shifts	886

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ADF monomer	1

Entities:

Entity 1, ADF monomer 150 residues - Formula weight is not available

1	MET	ALA	ILE	SER	GLY	VAL	THR	LEU	GLU	GLU
2	SER	VAL	ARG	GLY	ALA	ILE	ASP	ASP	LEU	ARG
3	MET	LYS	LYS	SER	ARG	TYR	VAL	MET	MET	CYS
4	ILE	GLY	ALA	ASP	GLY	LYS	LYS	ILE	GLU	VAL
5	THR	GLU	VAL	GLY	GLU	ARG	SER	VAL	ASN	TYR
6	THR	ASP	LEU	LYS	GLU	LYS	PHE	SER	THR	GLU
7	LYS	PRO	CYS	TYR	VAL	ALA	PHE	ASP	PHE	GLU
8	TYR	ASN	ASP	ALA	GLY	SER	LYS	ARG	GLU	LYS
9	LEU	ILE	LEU	ILE	GLN	TRP	ILE	PRO	ASP	THR
10	ALA	ARG	PRO	ARG	GLU	LYS	MET	MET	TYR	SER
11	ALA	SER	ARG	ASP	ALA	LEU	SER	SER	VAL	SER
12	GLU	GLY	TYR	LEU	PRO	ILE	GLN	ALA	ASN	ASP
13	GLU	SER	GLY	LEU	ASP	ALA	GLU	GLU	ILE	ILE
14	ARG	LYS	VAL	ARG	LEU	HIS	ARG	SER	VAL	ALA
15	ALA	ALA	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: Actin Depolymerzing Factor, [U-13C; U-15N], 1.5 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; DTT 1 mM; D2O 5%; AEBSF protease inhibitor 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CARA vCara_1.8.3_linux [4], Bartels et al., Cornilescu, Delaglio and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Keller and Wuthrich - chemical shift assignment, chemical shift assignment, processing

NMR spectrometers:

Varian UnityINOVA 600 MHz

PDB	2KVK
EMBL	CAM69611 CBZ12381 CBZ35776
GB	AAY99389
REF	XP_001466572 XP_003722124 XP_003862469

1	MET	ALA	ILE	SER	GLY	VAL	THR	LEU	GLU	GLU
2	SER	VAL	ARG	GLY	ALA	ILE	ASP	ASP	LEU	ARG
3	MET	LYS	LYS	SER	ARG	TYR	VAL	MET	MET	CYS
4	ILE	GLY	ALA	ASP	GLY	LYS	LYS	ILE	GLU	VAL
5	THR	GLU	VAL	GLY	GLU	ARG	SER	VAL	ASN	TYR
6	THR	ASP	LEU	LYS	GLU	LYS	PHE	SER	THR	GLU
7	LYS	PRO	CYS	TYR	VAL	ALA	PHE	ASP	PHE	GLU
8	TYR	ASN	ASP	ALA	GLY	SER	LYS	ARG	GLU	LYS
9	LEU	ILE	LEU	ILE	GLN	TRP	ILE	PRO	ASP	THR
10	ALA	ARG	PRO	ARG	GLU	LYS	MET	MET	TYR	SER
11	ALA	SER	ARG	ASP	ALA	LEU	SER	SER	VAL	SER
12	GLU	GLY	TYR	LEU	PRO	ILE	GLN	ALA	ASN	ASP
13	GLU	SER	GLY	LEU	ASP	ALA	GLU	GLU	ILE	ILE
14	ARG	LYS	VAL	ARG	LEU	HIS	ARG	SER	VAL	ALA
15	ALA	ALA	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	ALA	ILE	SER	GLY	VAL	THR	LEU	GLU	GLU
2	SER	VAL	ARG	GLY	ALA	ILE	ASP	ASP	LEU	ARG
3	MET	LYS	LYS	SER	ARG	TYR	VAL	MET	MET	CYS
4	ILE	GLY	ALA	ASP	GLY	LYS	LYS	ILE	GLU	VAL
5	THR	GLU	VAL	GLY	GLU	ARG	SER	VAL	ASN	TYR
6	THR	ASP	LEU	LYS	GLU	LYS	PHE	SER	THR	GLU
7	LYS	PRO	CYS	TYR	VAL	ALA	PHE	ASP	PHE	GLU
8	TYR	ASN	ASP	ALA	GLY	SER	LYS	ARG	GLU	LYS
9	LEU	ILE	LEU	ILE	GLN	TRP	ILE	PRO	ASP	THR
10	ALA	ARG	PRO	ARG	GLU	LYS	MET	MET	TYR	SER
11	ALA	SER	ARG	ASP	ALA	LEU	SER	SER	VAL	SER
12	GLU	GLY	TYR	LEU	PRO	ILE	GLN	ALA	ASN	ASP
13	GLU	SER	GLY	LEU	ASP	ALA	GLU	GLU	ILE	ILE
14	ARG	LYS	VAL	ARG	LEU	HIS	ARG	SER	VAL	ALA
15	ALA	ALA	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS