BMRB Entry 15552

Title:
Beta3 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments
Deposition date:
2007-11-09
Original release date:
2008-01-09
Authors:
Anthis, Nicholas; Wegener, Kate; Campbell, Iain
Citation:

Citation: Oxley, Camilla; Anthis, Nicholas; Lowe, Edward; Vakonakis, Ioannis; Campbell, Iain; Wegener, Kate. "An integrin phosphorylation switch: the effect of beta3 integrin tail phosphorylation on DOK1 and talin binding"  J. Biol. Chem. 283, 5420-5426 (2008).
PubMed: 18156175

Assembly members:

Assembly members:
beta3_integrin, polymer, 49 residues, 5730 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b (modified)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta3_integrin: GPKLLITIHDRKEFAKFEEE RARAKWDTANNPLYKEATST FTNITYRGT

Data sets:
Data typeCount
15N chemical shifts104
1H chemical shifts356

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta3 integrin1

Entities:

Entity 1, beta3 integrin 49 residues - 5730 Da.

This is the cytoplasmic tail of the human beta3 integrin. The first two residues (GP) are an artifact of 3C protease cleavage.

1   GLYPROLYSLEULEUILETHRILEHISASP
2   ARGLYSGLUPHEALALYSPHEGLUGLUGLU
3   ARGALAARGALALYSTRPASPTHRALAASN
4   ASNPROLEUTYRLYSGLUALATHRSERTHR
5   PHETHRASNILETHRTYRARGGLYTHR

Samples:

pH_4.0sample: beta3 integrin, [U-15N], 1 mM; D2O, [U-2H], 5%; DSS 100 uM; acetic acid 20 mM; D2O 5%; H20 95%

pH_6.1sample: beta3 integrin, [U-15N], 50 uM; DSS 100 uM; DTT 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; D2O 5%; H20 95%

pH_4.0: pH: 4; pressure: 1 atm; temperature: 298 K

pH_6.1: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCpH_4.0sampleisotropicpH_4.0
3D 1H-15N NOESYpH_4.0sampleisotropicpH_4.0
3D 1H-15N TOCSYpH_4.0sampleisotropicpH_4.0
2D 1H-15N HSQCpH_6.1sampleisotropicpH_6.1

Software:

SPARKY vVersion 3.111, Goddard - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection

NMR spectrometers:

  • home-built n/a 500 MHz
  • home-built n/a 600 MHz

Related Database Links:

BMRB 17930 17932
PDB
DBJ BAE34283 BAG62151 BAJ17755
EMBL CAD29521
GB AAA35927 AAA52589 AAA52600 AAA60122 AAA67537
REF NP_000203 NP_001003162 NP_001075271 NP_001075535 NP_001193419
SP O54890 P05106
TPG DAA18403
AlphaFold O54890 P05106

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks