BMRB Entry 15552

Name: Beta3 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments
Published: 2008-01-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15552

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Beta3 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments

Deposition date:

2007-11-09

Original release date:

2008-01-09

Authors:

Anthis, Nicholas; Wegener, Kate; Campbell, Iain

Citation:

Citation: Oxley, Camilla; Anthis, Nicholas; Lowe, Edward; Vakonakis, Ioannis; Campbell, Iain; Wegener, Kate. "An integrin phosphorylation switch: the effect of beta3 integrin tail phosphorylation on DOK1 and talin binding" J. Biol. Chem. 283, 5420-5426 (2008).
PubMed: 18156175

Assembly members:

Assembly members:
beta3_integrin, polymer, 49 residues, 5730 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b (modified)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta3_integrin: GPKLLITIHDRKEFAKFEEE RARAKWDTANNPLYKEATST FTNITYRGT

Data sets:

assigned_chemical_shifts
- 1H_15N_assignments_pH_4.0
- H-N_assignments_pH_6.1

Data type	Count
15N chemical shifts	104
1H chemical shifts	356

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta3 integrin	1

Entities:

Entity 1, beta3 integrin 49 residues - 5730 Da.

This is the cytoplasmic tail of the human beta3 integrin. The first two residues (GP) are an artifact of 3C protease cleavage.

1

GLY

PRO

LYS

LEU

ILE

THR

ILE

HIS

ASP

2

ARG

LYS

GLU

PHE

ALA

LYS

PHE

GLU

3

ARG

ALA

ARG

ALA

LYS

TRP

ASP

THR

ALA

ASN

4

ASN

PRO

LEU

TYR

LYS

GLU

ALA

THR

SER

THR

5

PHE

THR

ASN

ILE

THR

TYR

ARG

GLY

THR

Samples:

pH_4.0sample: beta3 integrin, [U-15N], 1 mM; D2O, [U-2H], 5%; DSS 100 uM; acetic acid 20 mM; D2O 5%; H20 95%

pH_6.1sample: beta3 integrin, [U-15N], 50 uM; DSS 100 uM; DTT 1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; D2O 5%; H20 95%

pH_4.0: pH: 4; pressure: 1 atm; temperature: 298 K

pH_6.1: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	pH_4.0sample	isotropic	pH_4.0
3D 1H-15N NOESY	pH_4.0sample	isotropic	pH_4.0
3D 1H-15N TOCSY	pH_4.0sample	isotropic	pH_4.0
2D 1H-15N HSQC	pH_6.1sample	isotropic	pH_6.1

Software:

SPARKY vVersion 3.111, Goddard - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Omega Spectrometer Operating Software vBeta 6.0.3b2, GE - collection

NMR spectrometers:

home-built n/a 500 MHz
home-built n/a 600 MHz

BMRB	17930 17932
PDB	1KUP 1KUZ 1M8O 1S4X 2KNC 2KV9 2LJD 2LJF 2MTP
DBJ	BAE34283 BAG62151 BAJ17755
EMBL	CAD29521
GB	AAA35927 AAA52589 AAA52600 AAA60122 AAA67537
REF	NP_000203 NP_001003162 NP_001075271 NP_001075535 NP_001193419
SP	O54890 P05106
TPG	DAA18403
AlphaFold	O54890 P05106