BMRB Entry 15551

Name: Backbone 1H and 15N Chemical Shift Assignments for Dok1 PTB domain
Published: 2008-01-09

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15551

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H and 15N Chemical Shift Assignments for Dok1 PTB domain

Deposition date:

2007-11-09

Original release date:

2008-01-09

Authors:

Oxley, Camilla; Vakonakis, Ioannis; Wegener, Kate; Campbell, Iain

Citation:

Citation: Oxley, Camilla; Anthis, Nicholas; Lowe, Edward; Vakonakis, Ioannis; Campbell, Iain; Wegener, Kate. "An integrin phosphorylation switch: the effect of beta3 integrin tail phosphorylation on DOK1 and talin binding." J. Biol. Chem. 283, 5420-5426 (2008).
PubMed: 18156175

Assembly members:

Assembly members:
Dok1, polymer, 108 residues, 12087.7 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX 6P2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dok1: GPLGSQFWVTVQRTEAAERC GLHGSYVLRVEAERLTLLTV GAQSQILEPLLSWWYTLLRR YGRDKVMFSFEAGRRCPSGP GTFTFQTAQGNDIFQAVETA IHRQKAQG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	113
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Dok1	1

Entities:

Entity 1, Dok1 108 residues - 12087.7 Da.

1

GLY

PRO

LEU

GLY

SER

GLN

PHE

TRP

VAL

THR

2

VAL

GLN

ARG

THR

GLU

ALA

GLU

ARG

CYS

3

GLY

LEU

HIS

GLY

SER

TYR

VAL

LEU

ARG

VAL

4

GLU

ALA

GLU

ARG

LEU

THR

LEU

THR

VAL

5

GLY

ALA

GLN

SER

GLN

ILE

LEU

GLU

PRO

LEU

6

LEU

SER

TRP

TYR

THR

LEU

ARG

7

TYR

GLY

ARG

ASP

LYS

VAL

MET

PHE

SER

PHE

8

GLU

ALA

GLY

ARG

CYS

PRO

SER

GLY

PRO

9

GLY

THR

PHE

THR

PHE

GLN

THR

ALA

GLN

GLY

10

ASN

ASP

ILE

PHE

GLN

ALA

VAL

GLU

THR

ALA

11

ILE

HIS

ARG

GLN

LYS

ALA

GLN

GLY

Samples:

sample_1: Dok1 (pH 6.1), [U-100% 13C; U-100% 15N], 1 mM; PBS 50 mM; NaCl 100 mM; DTT 10 mM; D2O 5%; Azide 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Home build Home build 600 MHz

BMRB	19672
PDB	2V76
DBJ	BAD96603 BAG51595 BAG58893 BAJ17765
GB	AAB88182 AAC51127 AAI14441 AAX93224 AIC48651
REF	NP_001184189 NP_001372 XP_001111248 XP_001161802 XP_002757635
SP	Q99704
AlphaFold	Q99704