BMRB Entry 15545

Name: 1H 13C and 15N chemical shift for the human FLN29
Published: 2008-01-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15545

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H 13C and 15N chemical shift for the human FLN29

Deposition date:

2007-11-05

Original release date:

2008-01-17

Authors:

Sauve, Simon; Gingras, Genevieve; Aubin, Remy; Hodgson, Derek; Aubin, Yves

Citation:

Citation: Sauve, Simon; Gingras, Genevieve; Aubin, Remy; Hodgson, Derek; Aubin, Yves. "NMR assignment of the N-terminal TRAF-like RING zinc finger domain of human FLN29" Biomol. NMR Assignments 2, 33-36 (2008).
PubMed: 19636918

Assembly members:

Assembly members:
FLN29, polymer, 141 residues, 16218.7 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15b10

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FLN29: GMAEFLDDQETRLCDNCKKE IPVFNFTIHEIHCQRNIGMC PTCKEPFPKSDMETHMAAEH CQVTCKCNKKLEKRLLKKHE ETECPLRLAVCQHCDLELSI LKLKEHEDYCGARTELCGNC GRNVLVKDLKTHPEVCGREG E

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	544
15N chemical shifts	136
1H chemical shifts	635

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FLN29	1
2	Zinc ion, 1	2
3	Zinc ion, 2	2
4	Zinc ion, 3	2
5	Zinc ion, 4	2

Entities:

Entity 1, FLN29 141 residues - 16218.7 Da.

The glycine residue in position 1 results from the TEV protease cleavage of the Histidine tag and is not part of the native N-terminal FLN29 sequence

1

GLY

MET

ALA

GLU

PHE

LEU

ASP

GLN

GLU

2

THR

ARG

LEU

CYS

ASP

ASN

CYS

LYS

GLU

3

ILE

PRO

VAL

PHE

ASN

PHE

THR

ILE

HIS

GLU

4

ILE

HIS

CYS

GLN

ARG

ASN

ILE

GLY

MET

CYS

5

PRO

THR

CYS

LYS

GLU

PRO

PHE

PRO

LYS

SER

6

ASP

MET

GLU

THR

HIS

MET

ALA

GLU

HIS

7

CYS

GLN

VAL

THR

CYS

LYS

CYS

ASN

LYS

8

LEU

GLU

LYS

ARG

LEU

LYS

HIS

GLU

9

GLU

THR

GLU

CYS

PRO

LEU

ARG

LEU

ALA

VAL

10

CYS

GLN

HIS

CYS

ASP

LEU

GLU

LEU

SER

ILE

11

LEU

LYS

LEU

LYS

GLU

HIS

GLU

ASP

TYR

CYS

12

GLY

ALA

ARG

THR

GLU

LEU

CYS

GLY

ASN

CYS

13

GLY

ARG

ASN

VAL

LEU

VAL

LYS

ASP

LEU

LYS

14

THR

HIS

PRO

GLU

VAL

CYS

GLY

ARG

GLU

GLY

15

GLU

Entity 2, Zinc ion, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: FLN29, [U-99% 13C; U-99% 15N], 200 uM; sodium phosphate 20 mM; zinc chloride 50 uM; DTT 1 mM; D2O 5%

sample_2: FLN29, [U-100% 13C; U-100% 15N], 400 uM; sodium phosphate 20 mM; zinc chloride 50 uM; DTT 1 mM; D2O 5%

sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D C(CO)NH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D C(CO)NH-TOCSY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v7.0.16, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

Smartnotebook v5.0.6, Brian Sykes Laboratory - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Varian INOVA 600 MHz
Bruker Avance III 600 MHz

DBJ	BAA22541 BAE00351 BAF84020 BAI46493
GB	AAH03553 ABM83456 ABM86667 AIC50745 EAW97995
REF	NP_001137378 NP_001253436 NP_001271978 NP_006691 XP_002753069
SP	O14545 Q4R970
AlphaFold	O14545 Q4R970