BMRB Entry 15539

Title:
Backbone 1H, 13C, and 15N NMR assignments for the Cyanothece 51142 protein A1KYE3 - a protein in the DUF683 family associated with nitrogen fixation.
Deposition date:
2007-10-28
Original release date:
2008-01-17
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Sofia, Heidi. "Backbone 1H, 13C, and 15N NMR assignments for the Cyanothece 51142 protein cce_0567: a protein associated with nitrogen fixation in the DUF683 family"  Biomol. NMR Assignments 2, 25-28 (2008).
PubMed: 19636916

Assembly members:

Assembly members:
DUF683_containing_polypeptide, polymer, 81 residues, 37000 Da.

Natural source:

Natural source:   Common Name: Cyanothece 51142   Taxonomy ID: 43989   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Cyanothece Cyanothece sp. ATCC 51142

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DUF683_containing_polypeptide: GSHMTVATDNNPTPEAVADL KKKVRKLNSKAGQMKMDLHD LAEGLPTDYENLVETAEKTY EIFRELDQLKKKLNIWEETL K

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts75
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Polypeptide 11
2Polypeptide 21
3Polypeptide 31
4Polypeptide 41

Entities:

Entity 1, Polypeptide 1 81 residues - 37000 Da.

Residues 1-3 remain after cleavage with thrombin. Fourth residue is M1.

1   GLYSERHISMETTHRVALALATHRASPASN
2   ASNPROTHRPROGLUALAVALALAASPLEU
3   LYSLYSLYSVALARGLYSLEUASNSERLYS
4   ALAGLYGLNMETLYSMETASPLEUHISASP
5   LEUALAGLUGLYLEUPROTHRASPTYRGLU
6   ASNLEUVALGLUTHRALAGLULYSTHRTYR
7   GLUILEPHEARGGLULEUASPGLNLEULYS
8   LYSLYSLEUASNILETRPGLUGLUTHRLEU
9   LYS

Samples:

sample_1: sodium chloride 500 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.1 mM; DUF683 containing polypeptide, [U-100% 13C; U-100% 15N], 1 ± 0.2 mM

sample_2: sodium chloride 500 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.1 mM; DUF683 containing polypeptide, [U-100% 13C; U-100% 15N; 90% 2H], 1 ± 0.2 mM

sample_3: sodium chloride 500 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.1 mM; DUF683 containing polypeptide, [U-15N]-His, 0.1 ± 0.2 mM

sample_4: sodium chloride 500 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.1 mM; DUF683 containing polypeptide, [U-15N]-Leu, 0.1 ± 0.2 mM

sample_5: sodium chloride 500 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.1 mM; DUF683 containing polypeptide, [U-15N]-Lys, 0.1 ± 0.2 mM

sample_6: sodium chloride 500 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.1 mM; DUF683 containing polypeptide, [U-15N]-Glu, 0.1 ± 0.2 mM

sample_7: sodium chloride 500 ± 2 mM; TRIS 20 ± 0.5 mM; DTT 1 ± 0.1 mM; DUF683 containing polypeptide, [U-15N]-Val, 0.1 ± 0.2 mM

sample_conditions_1: ionic strength: 521 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1
2D 1H-15N HSQCsample_7isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

FELIX v95, Accelrys Software Inc. - data analysis, peak picking, processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UnityPlus 500 MHz
  • Varian INOVA 750 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAW56996 ACB49918
REF WP_009546647

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks