BMRB Entry 15527

Name: 1H, 13C, and 31P Chemical Shift Assignments for 14-mer Base Pair Non-self Complementary DNA Duplex ( Mbp1_14) which Contains the Consensus Binding Site of the Yeast Transcription Factor Mbp-1
Published: 2009-06-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15527

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 31P Chemical Shift Assignments for 14-mer Base Pair Non-self Complementary DNA Duplex ( Mbp1_14) which Contains the Consensus Binding Site of the Yeast Transcription Factor Mbp-1

Deposition date:

2007-10-15

Original release date:

2009-06-10

Authors:

Chernatynskaya, Anna; Lane, Andrew

Citation:

Citation: Deleeuw, Lynn; Chernatynskaya, Anna; Lane, Andrew. "Structural analysis of the DNA target site and its interaction with Mbp1" Org. Biomol. Chem. ., .-..

Assembly members:

Assembly members:
DNA_Strand1, polymer, 14 residues, Formula weight is not available
DNA_Strand2, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: obtained from a vendor Vector: Integrated DNA Technologies, Inc.

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_Strand1: CTTACGCGTCATTG
DNA_Strand2: CAATGACGCGTAAG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	116
1H chemical shifts	227
31P chemical shifts	25

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNA Starnd1	1
2	DNA Strand2	2

Entities:

Entity 1, DNA Starnd1 14 residues - Formula weight is not available

1

DC

DT

DA

DC

DG

DC

DG

DT

DC

2

DA

DT

DG

Entity 2, DNA Strand2 14 residues - Formula weight is not available

1

DC

DA

DT

DG

DA

DC

DG

DC

DG

2

DT

DA

DG

Samples:

sample_1: sodium phosphate 10 mM; sodium chloride 100 mM; Mbp1_14 1.2 mM; H2O 100%

sample_2: sodium phosphate 10 mM; sodium chloride 100 mM; Mbp1_14 1.2 mM; H2O 90%; D2O 10%

sample_3: sodium phosphate 10 mM; sodium chloride 100 mM; Mbp1_14, [U-13C], 0.4 mM; Mbp1_14 0.4 mM; H2O 100%

sample_4: sodium phosphate 10 mM; sodium chloride 100 mM; Mbp1_14, [U-13C], 0.4 mM; Mbp1_14 0.4 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7; temperature: 303 K

sample_conditions_2: pH: 7; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
WGNOESY	sample_2	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-31P HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C NOESY-HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1

Software:

VNMR v6.1, Varian - processing

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz