BMRB Entry 15520

Name: SOLUTION STRUCTURE OF ENGRAILED HOMEODOMAIN L16A MUTANT
Published: 2007-10-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15520

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF ENGRAILED HOMEODOMAIN L16A MUTANT

Deposition date:

2007-10-12

Original release date:

2007-10-16

Authors:

Religa, Tomasz; Markson, Joseph; Mayor, Ugo; Freund, Stefan; Fersht, Alan

Citation:

Citation: Religa, Tomasz; Markson, Joseph; Mayor, Ugo; Freund, Stefan; Fersht, Alan. "Solution Structure of a Protein Denatured State and Folding Intermediate." Nature 437, 1053-1056 (2005).
PubMed: 16222301

Assembly members:

Assembly members:
SEGMENTATION_POLARITY_HOMEOBOX_PROTEIN_ENGRAILED, polymer, 61 residues, 7337.3 Da.

Natural source:

Natural source: Common Name: FRUIT FLY Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: DROSOPHILA MELANOGASTER

Experimental source:

Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI Vector: PRSET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SEGMENTATION_POLARITY_HOMEOBOX_PROTEIN_ENGRAILED: GDEKRPRTAFSSEQLARAKR EFNENRYLTERRRQQLSSEL GLNEAQIKIWFQNKRAKIKK S

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
coupling_constants
- coupling_constant_list_1

Data type	Count
13C chemical shifts	163
15N chemical shifts	58
1H chemical shifts	454
coupling constants	51

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED	1

Entities:

Entity 1, SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED 61 residues - 7337.3 Da.

1

GLY

ASP

GLU

LYS

ARG

PRO

ARG

THR

ALA

PHE

2

SER

GLU

GLN

LEU

ALA

ARG

ALA

LYS

ARG

3

GLU

PHE

ASN

GLU

ASN

ARG

TYR

LEU

THR

GLU

4

ARG

GLN

LEU

SER

GLU

LEU

5

GLY

LEU

ASN

GLU

ALA

GLN

ILE

LYS

ILE

TRP

6

PHE

GLN

ASN

LYS

ARG

ALA

LYS

ILE

LYS

7

SER

Samples:

15N: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-15N], 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

15N_PheTyr-H: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-15N; U-2H] [U-15N; U-1H]-Phe,Trp, 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

15N_U-D: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-15N; U-2H], 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

13C15N: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-13C; U-15N], 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

sample_conditions_1: ionic strength: 150 mM; pH: 5.7; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	15N	isotropic	sample_conditions_1
3D_15N-SEPARATED_ NOESY	15N_PheTyr-H	isotropic	sample_conditions_1
HSQC-NOESY-HSQC, 600MS MIXING TIME	15N_U-D	isotropic	sample_conditions_1
3D_13C-SEPARATED_ NOESY	13C15N	isotropic	sample_conditions_1
3D HNHA	15N	isotropic	sample_conditions_1

Software:

NMRPIPE 2004-04-12 v2004-04-12, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - structure solution

SPARKY v3.106, Goddard - structure solution

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

BRUKER DRX 800 MHz
BRUKER DRX 500 MHz

PDB	2P81 1ZTR 1ENH 1ENH 1HDD 2JWT 2P81 3HDD
BMRB	15536
DBJ	BAN82729 BAN82730 BAN82731
EMBL	CAA28436
GB	AAA65478 AAF58639 AAL39593 AAM68711 ACL84189
REF	NP_523700 NP_725059 XP_001958778 XP_001976053 XP_002006437
SP	P02836 P09145
AlphaFold	P02836 P09145